Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/122541
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Type: Journal article
Title: A biophotonic approach to measure pH in small volumes in vitro: quantifiable differences in metabolic flux around the cumulus-oocyte-complex (COC)
Author: McLennan, H.J.
Saini, A.
Sylvia, G.M.
Schartner, E.P.
Dunning, K.R.
Purdey, M.S.
Monro, T.M.
Abell, A.D.
Thompson, J.G.
Citation: Journal of Biophotonics, 2020; 13(3):e201960038-1-e201960038-9
Publisher: Wiley
Issue Date: 2020
ISSN: 1864-063X
1864-0648
Statement of
Responsibility: 
Hanna J. McLennan, Avishkar Saini, Georgina M. Sylvia, Erik P. Schartner, Kylie R. Dunning, Malcolm S. Purdey, Tanya M. Monro, Andrew D. Abell, Jeremy G. Thompson
Abstract: Unfertilised eggs (oocytes) release chemical biomarkers into the medium surrounding them. This provides an opportunity to monitor cell health and development during assisted reproductive processes if detected in a non-invasive manner. Here we report the measurement of pH using an optical fibre probe, OFP1, in 5 μL drops of culture medium containing single mouse cumulus-oocyte-complexes (COCs). This allowed for the detection of statistically significant differences in pH between COCs in culture medium with no additives and those incubated with either a chemical (cobalt chloride) or hormonal treatment (follicle stimulating hormone); both of which serve to induce the release of lactic acid into the medium immediately surrounding the COC. Importantly, OFP1 was shown to be cell-safe with no inherent cell toxicity or light-induced phototoxicity indicated by negative DNA damage staining. Pre-measurement photobleaching of the probe reduced fluorescence signal variability, providing improved measurement precision (0.01 - 0.05 pH units) compared to previous studies. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction. This article is protected by copyright. All rights reserved.
Keywords: biomarker; biophotonic; cumulus oocyte complex (COC); fluorescence sensing; lactic acid; microenvironment; optical fibre; pH sensing
Rights: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
RMID: 1000005069
DOI: 10.1002/jbio.201960038
Grant ID: http://purl.org/au-research/grants/arc/LP110200736
http://purl.org/au-research/grants/arc/CE140100003
http://purl.org/au-research/grants/arc/FL130100044
http://purl.org/au-research/grants/nhmrc/1077694
Appears in Collections:Medicine publications

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