Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/122541
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | McLennan, H.J. | - |
dc.contributor.author | Saini, A. | - |
dc.contributor.author | Sylvia, G.M. | - |
dc.contributor.author | Schartner, E.P. | - |
dc.contributor.author | Dunning, K.R. | - |
dc.contributor.author | Purdey, M.S. | - |
dc.contributor.author | Monro, T.M. | - |
dc.contributor.author | Abell, A.D. | - |
dc.contributor.author | Thompson, J.G. | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Journal of Biophotonics, 2020; 13(3):e201960038-1-e201960038-9 | - |
dc.identifier.issn | 1864-063X | - |
dc.identifier.issn | 1864-0648 | - |
dc.identifier.uri | http://hdl.handle.net/2440/122541 | - |
dc.description.abstract | Unfertilised eggs (oocytes) release chemical biomarkers into the medium surrounding them. This provides an opportunity to monitor cell health and development during assisted reproductive processes if detected in a non-invasive manner. Here we report the measurement of pH using an optical fibre probe, OFP1, in 5 μL drops of culture medium containing single mouse cumulus-oocyte-complexes (COCs). This allowed for the detection of statistically significant differences in pH between COCs in culture medium with no additives and those incubated with either a chemical (cobalt chloride) or hormonal treatment (follicle stimulating hormone); both of which serve to induce the release of lactic acid into the medium immediately surrounding the COC. Importantly, OFP1 was shown to be cell-safe with no inherent cell toxicity or light-induced phototoxicity indicated by negative DNA damage staining. Pre-measurement photobleaching of the probe reduced fluorescence signal variability, providing improved measurement precision (0.01 - 0.05 pH units) compared to previous studies. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction. This article is protected by copyright. All rights reserved. | - |
dc.description.statementofresponsibility | Hanna J. McLennan, Avishkar Saini, Georgina M. Sylvia, Erik P. Schartner, Kylie R. Dunning, Malcolm S. Purdey, Tanya M. Monro, Andrew D. Abell, Jeremy G. Thompson | - |
dc.language.iso | en | - |
dc.publisher | Wiley | - |
dc.relation.isreplacedby | 2440/129423 | - |
dc.relation.isreplacedby | http://hdl.handle.net/2440/129423 | - |
dc.rights | © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim | - |
dc.source.uri | http://dx.doi.org/10.1002/jbio.201960038 | - |
dc.subject | biomarker | - |
dc.subject | biophotonic | - |
dc.subject | cumulus oocyte complex (COC) | - |
dc.subject | fluorescence sensing | - |
dc.subject | lactic acid | - |
dc.subject | microenvironment | - |
dc.subject | optical fibre | - |
dc.subject | pH sensing | - |
dc.title | A biophotonic approach to measure pH in small volumes in vitro: quantifiable differences in metabolic flux around the cumulus-oocyte-complex (COC) | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1002/jbio.201960038 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/LP110200736 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/CE140100003 | - |
dc.relation.grant | http://purl.org/au-research/grants/arc/FL130100044 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/1077694 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | McLennan, H.J. [0000-0002-2058-2488] | - |
dc.identifier.orcid | Saini, A. [0000-0002-2221-7476] | - |
dc.identifier.orcid | Sylvia, G.M. [0000-0002-1258-1864] | - |
dc.identifier.orcid | Schartner, E.P. [0000-0003-1669-4302] | - |
dc.identifier.orcid | Dunning, K.R. [0000-0002-0462-6479] | - |
dc.identifier.orcid | Purdey, M.S. [0000-0002-5063-8972] | - |
dc.identifier.orcid | Abell, A.D. [0000-0002-0604-2629] | - |
dc.identifier.orcid | Thompson, J.G. [0000-0003-4941-7731] | - |
Appears in Collections: | Aurora harvest 4 Medicine publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_122541.pdf | Accepted version | 1.95 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.