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Type: Journal article
Title: Stathmin levels alter PTPN14 expression and impact neuroblastoma cell migration
Author: Po uha, S.T.
Le Grand, M.
Brandl, M.B.
Gifford, A.J.
Goodall, G.J.
Khew-Goodall, Y.
Kavallaris, M.
Citation: British Journal of Cancer, 2020; 122(3):434-444
Publisher: Nature
Issue Date: 2020
ISSN: 0007-0920
Statement of
Sela T. Po, uha, Marion Le Grand, Miriam B. Brandl, Andrew J. Gifford, Gregory J. Goodall, Yeesim Khew-Goodall and Maria Kavallaris
Abstract: Background: Stathmin mediates cell migration and invasion in vitro, and metastasis in vivo. To investigate stathmin’s role on the metastatic process, we performed integrated mRNA–miRNA expression analysis to identify pathways regulated by stathmin. Methods: MiRNA and gene arrays followed by miRNA-target-gene integration were performed on stathmin-depleted neuroblastoma cells (CtrlshRNA vs. Stmn Seq2shRNA). The expression of the predicted target PTPN14 was evaluated by RT-qPCR, western blot and immunohistochemistry. Gene-silencing technology was used to assess the role of PTPN14 on proliferation, migration, invasion and signalling pathway. Results: Stathmin levels modulated the expression of genes and miRNA in neuroblastoma cells, leading to a deregulation of migration and invasion pathways. Consistent with gene array data, PTPN14 mRNA and protein expression were downregulated in stathmin- depleted neuroblastoma cells and xenografts. In two independent neuroblastoma cells, suppression of PTPN14 expression led to an increase in cell migration and invasion. PTPN14 and stathmin expression did not act in a feedback regulatory loop in PTPN14- depleted cells, suggesting a complex interplay of signalling pathways. The effect of PTPN14 on YAP pathway activation was cell-type dependent. Conclusions: Our findings demonstrate that stathmin levels can regulate PTPN14 expression, which can modulate neuroblastoma cell migration and invasion.
Keywords: Cell Line, Tumor
Mice, SCID
Neoplasm Invasiveness
Neoplasm Metastasis
RNA, Messenger
Neoplasm Transplantation
Signal Transduction
Cell Proliferation
Cell Movement
Gene Expression Regulation, Neoplastic
Protein Tyrosine Phosphatases, Non-Receptor
Rights: © The Author(s), under exclusive licence to Cancer Research UK 2019
DOI: 10.1038/s41416-019-0669-1
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