Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/122896
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Type: Journal article
Title: Single-dose vaccination with a hepatotropic adeno-associated virus efficiently localizes T cell immunity in the liver with the potential to confer rapid protection against Hepatitis C virus
Author: Mekonnen, Z.A.
Grubor-Bauk, B.
English, K.
Leung, P.
Masavuli, M.G.
Shrestha, A.C.
Bertolino, P.
Bowen, D.G.
Lloyd, A.R.
Gowans, E.J.
Wijesundara, D.K.
Citation: Journal of Virology, 2019; 93(19):e00202-19-1-e00202-19-19
Publisher: American Society for Microbiology
Issue Date: 2019
ISSN: 0022-538X
1098-5514
Statement of
Responsibility: 
Zelalem A. Mekonnen, Branka Grubor-Bauk, Kieran English, Preston Leung, Makutiro G. Masavuli, Ashish C. Shrestha, Patrick Bertolino, David G. Bowen, Andrew R. Lloyd, Eric J. Gowans, Danushka K. Wijesundara
Abstract: Hepatitis C virus (HCV) is a significant contributor to the global disease burden and development of an effective vaccine is required to eliminate HCV infections worldwide. CD4+ and CD8+ T cell immunity correlate with viral clearance in primary HCV infection, and intrahepatic CD8+ tissue-resident memory T (TRM) cells provide lifelong and rapid protection against hepatotropic pathogens. Consequently, we aimed to develop a vaccine to elicit HCV-specific CD4+ and CD8+ T cells, including CD8+ TRM cells in the liver given that HCV primarily infects hepatocytes. To achieve this we vaccinated wild-type BALB/c mice with a highly immunogenic cytolytic DNA vaccine encoding a model HCV (genotype 3a) non-structural protein 5B (NS5B) and a mutant perforin (pVAX-NS5B-PRF) as well as a recombinant adeno-associated virus (AAV) encoding NS5B (rAAV-NS5B). A novel fluorescent target array (FTA) was used to map immunodominant CD4+ T helper (TH) cell and cytotoxic CD8+ T cell epitopes of NS5B in vivo which were subsequently used to design a KdNS5B451-459 tetramer and analyse NS5B-specific T cell responses in vaccinated mice in vivo The data showed that intradermal prime/boost vaccination with pVAX-NS5B-PRF was effective in eliciting TH and cytotoxic CD8+ T cell responses, and intrahepatic CD8+ TRM cells, but a single intravenous dose of hepatotropic rAAV-NS5B was significantly more effective. As a T-cell based vaccine against HCV should ideally result in localised T cell responses in the liver this study describes primary observations in the context of HCV vaccination which can be used to achieve this goal. Importance: There are currently at least 71 million individuals with chronic HCV worldwide, and almost two million new infections annually. Although the advent of direct-acting antivirals (DAAs) offer highly effective therapy, considerable remaining challenges argue against reliance on DAAs for HCV elimination including high drug cost, poorly developed health infrastructure, low screening rates, and significant reinfection rates. Accordingly, development of an effective vaccine is crucial to HCV elimination. A HCV vaccine which elicits T cell immunity in the liver will be highly protective for the following reasons: 1) T cell responses against non-structural proteins of the virus are associated with clearance of primary infection, and 2) long-lived liver-resident T cells alone can protect against malaria infection of hepatocytes. Thus, in this study we exploit promising vaccination platforms to highlight strategies that can be used to evoke highly functional and long-lived T cell responses in the liver for protection against HCV.
Keywords: Adeno-associated virus vaccine; cytotoxic T cells; DNA vaccine; helper T cells; hepatitis C virus vaccine; liver immunity
Rights: Copyright © 2019 American Society for Microbiology. All Rights Reserved.
DOI: 10.1128/JVI.00202-19
Grant ID: http://purl.org/au-research/grants/nhmrc/1043067
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