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|dc.identifier.citation||Scientific Reports, 2019; 9(1):4398-1-4398-10||en|
|dc.description.abstract||Damage and degradation of articular cartilage leads to severe pain and loss of mobility. The development of new therapies for cartilage regeneration for monitoring their effect requires further study of cartilage, ideally at a molecular level and in a minimally invasive way. Hyperspectral microscopy is a novel technology which utilises endogenous fluorophores to non-invasively assess the molecular composition of cells and tissue. In this study, we applied hyperspectral microscopy to healthy bovine articular cartilage and osteoarthritic human articular cartilage to investigate its capacity to generate informative molecular data and characterise disease state and treatment effects. We successfully demonstrated label-free fluorescence identification of collagen type I and II - isolated in cartilage here for the first time and the co-enzymes free NADH and FAD which together give the optical redox ratio that is an important measure of metabolic activity. The intracellular composition of chondrocytes was also examined. Differences were observed in the molecular ratios within the superficial and transitional zones of the articular cartilage which appeared to be influenced by disease state and treatment. These findings show that hyperspectral microscopy could be useful for investigating the molecular underpinnings of articular cartilage degradation and repair. As it is non-invasive and non-destructive, samples can be repeatedly assessed over time, enabling true time-course experiments with in-depth molecular data. Additionally, there is potential for the hyperspectral approach to be adapted for patient examination to allow the investigation of cartilage state. This could be of advantage for assessment and diagnosis as well as treatment monitoring.||en|
|dc.description.statementofresponsibility||Saabah B. Mahbub, Anna Guller, Jared M. Campbell, Ayad G. Anwer, Martin E. Gosnell, Graham Vesey, Ewa M. Goldys||en|
|dc.rights||© The Author(s) 2019. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.||en|
|dc.subject||Cartilage, Articular; Animals; Cattle; Humans; Microscopy, Fluorescence; Middle Aged; Male; Optical Imaging; In Vitro Techniques||en|
|dc.title||Non-invasive monitoring of functional state of articular cartilage tissue with label-free unsupervised hyperspectral imaging||en|
|pubs.library.collection||Public Health publications||en|
|dc.identifier.orcid||Campbell, J.M. [0000-0003-0163-4251]||en|
|Appears in Collections:||Public Health publications|
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