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Type: Journal article
Title: Differential tissue expression of epitopes of the tetraspanin CD151 recognised by monoclonal antibodies
Author: Geary, S.
Cambareri, A.
Sincock, P.
Fitter, S.
Ashman, L.
Citation: Tissue Antigens: immune response genetics, 2001; 58(3):141-153
Publisher: Blackwell Munksgaard
Issue Date: 2001
ISSN: 0001-2815
Abstract: CD151, a member of the tetraspanin family of cell membrane proteins, is widely expressed in epithelial, endothelial and muscle cells as well as platelets and megakaryocytes. Several monoclonal antibodies recognising CD151 in transfected cells and immunoprecipitating typical bands of 28 and 32 kDa from cell lysates have been produced. Surprisingly, these antibodies show different patterns of staining on tissue sections and on haemopoietic cells. Here we show that these differences are at least in part due to masking of certain epitopes in integrin/CD151 complexes. These data have important implications for the use of monoclonal antibodies in studies of the distribution and function of CD151. Of six monoclonal antibodies from four laboratories, 11B1 was found to be the most reliable for detection of CD151 in different cell and tissue contexts.
Keywords: Muscles
Bone Marrow Cells
Cells, Cultured
Cell Line
Macromolecular Substances
Membrane Proteins
Integrin alpha3
Integrin alpha6
Antigens, CD
Antibodies, Monoclonal
Fluorescent Antibody Technique
Blotting, Western
Immunoenzyme Techniques
Precipitin Tests
Antibody Specificity
Tetraspanin 24
DOI: 10.1034/j.1399-0039.2001.580301.x
Appears in Collections:Aurora harvest 2
Ecology, Evolution and Landscape Science publications

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