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Type: Journal article
Title: Optimization of agitation speed in spinner flask for microcarrier structural integrity and expansion of induced pluripotent stem cells
Author: Gupta, P.
Ismadi, M.Z.
Verma, P.J.
Fouras, A.
Jadhav, S.
Bellare, J.
Hourigan, K.
Citation: Cytotechnology: international journal of cell culture and biotechnology, 2016; 68(1):45-59
Publisher: Springer
Issue Date: 2016
ISSN: 0920-9069
Statement of
Priyanka Gupta, Mohd-Zulhilmi Ismadi, Paul J. Verma, Andreas Fouras, Sameer Jadhav, Jayesh Bellare, Kerry Hourigan
Abstract: In recent times, the study and use of induced pluripotent stem cells (iPSC) have become important in order to avoid the ethical issues surrounding the use of embryonic stem cells. Therapeutic, industrial and research based use of iPSC requires large quantities of cells generated in vitro. Mammalian cells, including pluripotent stem cells, have been expanded using 3D culture, however current limitations have not been overcome to allow a uniform, optimized platform for dynamic culture of pluripotent stem cells to be achieved. In the current work, we have expanded mouse iPSC in a spinner flask using Cytodex 3 microcarriers. We have looked at the effect of agitation on the microcarrier survival and optimized an agitation speed that supports bead suspension and iPS cell expansion without any bead breakage. Under the optimized conditions, the mouse iPSC were able to maintain their growth, pluripotency and differentiation capability. We demonstrate that microcarrier survival and iPS cell expansion in a spinner flask are reliant on a very narrow range of spin rates, highlighting the need for precise control of such set ups and the need for improved design of more robust systems.
Keywords: Induced pluripotent stem cells
Spinner flask
Rights: © Springer Science+Business Media Dordrecht 2014.
DOI: 10.1007/s10616-014-9750-z
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Appears in Collections:Animal and Veterinary Sciences publications
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