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Type: Journal article
Title: VPAC₁ receptors regulate intestinal secretion and muscle contractility by activating cholinergic neurons in guinea pig jejunum
Other Titles: VPAC(1) receptors regulate intestinal secretion and muscle contractility by activating cholinergic neurons in guinea pig jejunum
Author: Fung, C.
Unterweger, P.
Parry, L.J.
Bornstein, J.C.
Foong, J.P.P.
Citation: American Journal of Physiology: Gastrointestinal and Liver Physiology, 2014; 306(9):G748-G758
Publisher: American Physiological Society
Issue Date: 2014
ISSN: 0193-1857
Statement of
Candice Fung, Petra Unterweger, Laura J. Parry, Joel C. Bornstein and Jaime P.P. Foong
Abstract: In the gastrointestinal tract, vasoactive intestinal peptide (VIP) is found exclusively within neurons. VIP regulates intestinal motility via neurally mediated and direct actions on smooth muscle and secretion by a direct mucosal action, and via actions on submucosal neurons. VIP acts via VPAC₁ and VPAC₂ receptors; however, the subtype involved in its neural actions is unclear. The neural roles of VIP and VPAC₁ receptors (VPAC₁R) were investigated in intestinal motility and secretion in guinea pig jejunum. Expression of VIP receptors across the jejunal layers was examined using RT-PCR. Submucosal and myenteric neurons expressing VIP receptor subtype VPAC₁ and/or various neurochemical markers were identified immunohistochemically. Isotonic muscle contraction was measured in longitudinal muscle-myenteric plexus preparations. Electrogenic secretion across mucosa-submucosa preparations was measured in Ussing chambers by monitoring short-circuit current. Calretinin⁺ excitatory longitudinal muscle motor neurons expressed VPAC₁R. Most cholinergic submucosal neurons, notably NPY⁺ secretomotor neurons, expressed VPAC₁R. VIP (100 nM) induced longitudinal muscle contraction that was inhibited by TTX (1 μM), PG97-269 (VPAC₁ antagonist; 1 μM), and hyoscine (10 μM), but not by hexamethonium (200 μM). VIP (50 nM)-evoked secretion was depressed by hyoscine or PG97-269 and involved a small TTX-sensitive component. PG97-269 and TTX combined did not further depress the VIP response observed in the presence of PG97-269 alone. We conclude that VIP stimulates ACh-mediated longitudinal muscle contraction via VPAC₁R on cholinergic motor neurons. VIP induces Cl(-) secretion directly via epithelial VPAC₁R and indirectly via VPAC₁R on cholinergic secretomotor neurons. No evidence was obtained for involvement of other neural VIP receptors.
Keywords: Vasoactive intestinal peptide; VPAC₁ receptor; motility; secretion; enteric
Rights: © 2014 the American Physiological Society
DOI: 10.1152/ajpgi.00416.2013
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Molecular and Biomedical Science publications

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