Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/123434
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Type: Journal article
Title: Transposon mutagenesis reveals cooperation of ETS family transcription factors with signaling pathways in erythro-megakaryocytic leukemia
Author: Tang, J.
Carmichael, C.
Shi, W.
Metcalf, D.
Ng, A.
Hyland, C.
Jenkins, N.
Copeland, N.
Howell, V.
Zhao, Z.
Smyth, G.
Kile, B.
Alexander, W.
Citation: Proceedings of the National Academy of Sciences of USA, 2013; 110(15):6091-6096
Publisher: National Academy of Sciences
Issue Date: 2013
ISSN: 0027-8424
1091-6490
Statement of
Responsibility: 
Jian Zhong Tang, Catherine L. Carmichael, Wei Shi, Donald Metcalf, Ashley P. Ng, Craig D. Hyland, Nancy A. Jenkins, Neal G. Copeland, Viive M. Howell, Zhizhuang Joe Zhao, Gordon K. Smyth, Benjamin T. Kile, and Warren S. Alexander
Abstract: To define genetic lesions driving leukemia, we targeted cre-dependent Sleeping Beauty (SB) transposon mutagenesis to the blood-forming system using a hematopoietic-selective vav 1 oncogene (vav1) promoter. Leukemias of diverse lineages ensued, most commonly lymphoid leukemia and erythroleukemia. The inclusion of a transgenic allele of Janus kinase 2 (JAK2)V617F resulted in acceleration of transposon-driven disease and strong selection for erythroleukemic pathology with transformation of bipotential erythro-megakaryocytic cells. The genes encoding the E-twenty-six (ETS) transcription factors Ets related gene (Erg) and Ets1 were the most common sites for transposon insertion in SB-induced JAK2V617F-positive erythroleukemias, present in 87.5% and 65%, respectively, of independent leukemias examined. The role of activated Erg was validated by reproducing erythroleukemic pathology in mice transplanted with fetal liver cells expressing translocated in liposarcoma (TLS)-ERG, an activated form of ERG found in human leukemia. Via application of SB mutagenesis to TLS-ERG-induced erythroid transformation, we identified multiple loci as likely collaborators with activation of Erg. Jak2 was identified as a common transposon insertion site in TLS-ERG-induced disease, strongly validating the cooperation between JAK2V617F and transposon insertion at the Erg locus in the JAK2V617F-positive leukemias. Moreover, loci expressing other regulators of signal transduction pathways were conspicuous among the common transposon insertion sites in TLS-ERG-driven leukemia, suggesting that a key mechanism in erythroleukemia may be the collaboration of lesions disturbing erythroid maturation, most notably in genes of the ETS family, with mutations that reduce dependence on exogenous signals.
Keywords: Transcription Factors
Rights: The author(s) retains copyright to individual PNAS articles, and the National Academy of Sciences of the United States of America (NAS) holds copyright to the collective work and retains an exclusive License to Publish these articles, except for open access articles submitted beginning September 2017.
DOI: 10.1073/pnas.1304234110
Grant ID: http://purl.org/au-research/grants/nhmrc/361646
http://purl.org/au-research/grants/nhmrc/1016647
http://purl.org/au-research/grants/nhmrc/490037
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