Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/123584
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Type: Journal article
Title: Conserved piRNA expression from a distinct set of piRNA cluster loci in eutherian mammals
Author: Chirn, G.
Rahman, R.
Sytnikova, Y.
Matts, J.
Zeng, M.
Gerlach, D.
Yu, M.
Berger, B.
Naramura, M.
Kile, B.
Lau, N.
Citation: PLoS Genetics, 2015; 11(11):e1005652-1-e1005652-26
Publisher: Public Library of Science
Issue Date: 2015
ISSN: 1553-7390
1553-7404
Statement of
Responsibility: 
Gung-wei Chirn, Reazur Rahman, Yuliya A. Sytnikova, Jessica A. Matts, Mei Zeng ... Benjamin T. Kile ... et al.
Abstract: The Piwi pathway is deeply conserved amongst animals because one of its essential functions is to repress transposons. However, many Piwi-interacting RNAs (piRNAs) do not base-pair to transposons and remain mysterious in their targeting function. The sheer number of piRNA cluster (piC) loci in animal genomes and infrequent piRNA sequence conservation also present challenges in determining which piC loci are most important for development. To address this question, we determined the piRNA expression patterns of piC loci across a wide phylogenetic spectrum of animals, and reveal that most genic and intergenic piC loci evolve rapidly in their capacity to generate piRNAs, regardless of known transposon silencing function. Surprisingly, we also uncovered a distinct set of piC loci with piRNA expression conserved deeply in Eutherian mammals. We name these loci Eutherian-Conserved piRNA cluster (ECpiC) loci. Supporting the hypothesis that conservation of piRNA expression across ~100 million years of Eutherian evolution implies function, we determined that one ECpiC locus generates abundant piRNAs antisense to the STOX1 transcript, a gene clinically associated with preeclampsia. Furthermore, we confirmed reduced piRNAs in existing mouse mutations at ECpiC-Asb1 and -Cbl, which also display spermatogenic defects. The Asb1 mutant testes with strongly reduced Asb1 piRNAs also exhibit up-regulated gene expression profiles. These data indicate ECpiC loci may be specially adapted to support Eutherian reproduction.
Keywords: RNA, Small Interfering
Rights: © 2015 Chirn et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 1000001812
DOI: 10.1371/journal.pgen.1005652
Grant ID: http://purl.org/au-research/grants/nhmrc/1016647
http://purl.org/au-research/grants/nhmrc/361646
Appears in Collections:Medical Sciences publications

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