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Type: Journal article
Title: Molecular identification and phylogenetic analysis of Lactobacillus and Bifidobacterium spp. isolated from gut of honeybees (Apis mellifera) from West Azerbaijan, Iran
Author: Sharifpour, M.
Mardani, K.
Ownagh, A.
Citation: Veterinary Research Forum, 2016; 7(4):287-294
Publisher: Urmia University Press
Issue Date: 2016
ISSN: 2008-8140
Statement of
Mohammad Farouq Sharifpour, Karim Mardani, Abdulghaffar Ownagh
Abstract: Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) and phylogenetic analysis were used for molecular identification of lactic acid bacteria (LABs) isolated from Apis mellifera. Eighteen honeybee workers were collected from three different apiaries in West Azerbaijan. LABs from the gut of honeybees were isolated and cultured using routine biochemical procedures. Genomic DNA was extracted from LABs and a fragment of 1540 bp in size of 16S rRNA gene was amplified. PCR products were digested using HinfI endonuclease and digested products with different RFLP patterns were subjected to nucleotide sequencing and phylogenetic analysis. The results revealed that Lactobacillus and Bifidobacteria spp. are were the most abundant LABs in honeybee gut. Phylogenetic analysis showed that both Lactobacillus and Bifidobacterium were closely clustered with high similarity percentage with the same bacteria isolated from honeybees’ gut elsewhere. It was concluded that LABs isolated from honeybees had low sequence divergence in comparison with LABs isolated from other sources such as dairy products.
Keywords: Apis mellifera; Honeybee; Lactic acid bacteria; Molecular identification; Phylogenetic analysis
Description: Available online: 15 December 2016
Rights: © 2016 Urmia University. All rights reserved. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
RMID: 1000018751
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Appears in Collections:Animal and Veterinary Sciences publications

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