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Type: Journal article
Title: A multiplexed microfluidic platform towards interrogating endocrine function: simultaneous sensing of extracellular Ca²⁺ and hormone
Other Titles: A multiplexed microfluidic platform towards interrogating endocrine function: simultaneous sensing of extracellular Ca(2+) and hormone
Author: Huang, W.
Wu, T.
Shallan, A.
Kostecki, R.
Rayner, C.K.
Priest, C.
Ebendorff-Heidepriem, H.
Zhao, J.
Citation: ACS sensors, 2020; 5(2):490-499
Publisher: American Chemical Society
Issue Date: 2020
ISSN: 2379-3694
Statement of
Weikun Huang, Tongzhi Wu, Aliaa Shallan, Roman Kostecki, Christopher K. Rayner, Craig Priest, Heike Ebendorff-Heidepriem, Jiangbo Zhao
Abstract: Extracellular Ca2+ ([Ca2+]ex) is an important regulator of various physiological and pathological functions, including intercellular communication for synchronized cellular activities (e.g., coordinated hormone secretion from endocrine tissues). Yet rarely is it possible to concurrently quantify the dynamic changes of [Ca2+]ex and related bioactive molecules with high accuracy and temporal resolution. This work aims to develop a multiplexed microfluidic platform to enable monitoring oscillatory [Ca2+]ex and hormone(s) in a biomimetic environment. To this end, a low-affinity fluorescent indicator, Rhod-5N, is identified as a suitable sensor for a range of [Ca2+]ex based on its demonstrated high sensitivity and selectivity to Ca2+ in biomedical samples, including human serum and cell culture medium. A microfluidic chip is devised to allow for immobilization of microscale subjects (analogous to biological tissues), precise control of the perfusion gradient at sites of interest, and integration of modalities for fluorescence measurement and enzyme-linked immunosorbent assay. As this analytical system is demonstrated to be viable to quantify the dynamic changes of Ca2+ (0.2-2 mM) and insulin (15-150 mU L-1) concurrently, with high temporal resolution, it has potential to provide key insights into the essential roles of [Ca2+]ex in the secretory function of endocrine tissues and to identify novel therapeutic targets for human diseases.
Keywords: Ca2+ sensors; hormone detection; microfluidics; multiplexed sensing; oscillatory secretion
Rights: © 2020 American Chemical Society
DOI: 10.1021/acssensors.9b02308
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Physics publications

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