Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/124659
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Type: Journal article
Title: The microbial abundance dynamics of the paediatric oral cavity before and after sleep
Author: Carlson-Jones, J.A.P.
Kontos, A.
Kennedy, J.
Martin, A.
Lushington, K.
McKerral, J.
Paterson, J.S.
Smith, R.J.
Dann, L.M.
Speck, P.
Mitchell, J.G.
Citation: Journal of Oral Microbiology, 2020; 12(1):1741254-1-1741254-12
Publisher: Taylor & Francis
Issue Date: 2020
ISSN: 2000-2297
2000-2297
Statement of
Responsibility: 
Jessica A.P. Carlson-Jones, Anna Kontos, Declan Kennedy, James Martin, Kurt Lushington, Jody McKerral, James S. Paterson, Renee J. Smith, Lisa M. Dann, Peter Speck and James G. Mitchell
Abstract: Objective: Microhabitats in the oral cavity differ in microbial taxonomy. However, abundance variations of bacterial and viral communities within these microhabitats are not fully understood. Aims and Hypothesis: To assess the spatial distribution and dynamics of the microbial abundances within 6 microhabitats of the oral cavity before and after sleep. We hypothesise that the abundance distributions of these microbial communities will differ among oral sites. Methods: Using flow cytometry, bacterial and virus-like particle (VLP) abundances were enumerated for 6 oral microhabitats before and after sleep in 10 healthy paediatric sleepers. Results: Bacterial counts ranged from 7.2 ± 2.8 × 10⁵ at the palate before sleep to 1.3 ± 0.2 × 10⁸ at the back of the tongue after sleep, a difference of 187 times. VLPs ranged from 1.9 ± 1.0 × 10⁶ at the palate before sleep to 9.2 ± 5.0 × 10⁷ at the back of the tongue after sleep, a difference of 48 times. Conclusion: The oral cavity is a dynamic numerically heterogeneous environment where microbial communities can increase by a count of 100 million during sleep. Quantification of the paediatric oral microbiome complements taxonomic diversity information to show how biomass varies and shifts in space and time.
Keywords: Oral microbiome; sleep; flow cytometry; bacteria; viruses
Rights: © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1080/20002297.2020.1741254
Grant ID: http://purl.org/au-research/grants/arc/DP150103018
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