Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/124677
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Type: Journal article
Title: Conversion of human urine-derived cells into neuron-like cells by small molecules
Author: Liu, D.
Rychkov, G.
Al-Hawwas, M.
Manaph, N.P.A.
Zhou, F.
Bobrovskaya, L.
Liao, H.
Zhou, X.-.F.
Citation: Molecular Biology Reports, 2020; 47(4):1-10
Publisher: Springer
Issue Date: 2020
ISSN: 0301-4851
1573-4978
Statement of
Responsibility: 
Donghui Liu, Grigori Rychkov, Mohammed Al‑Hawwas, Nimshitha Pavathuparambil Abdul Manaph, Fiona Zhou, Larisa Bobrovskaya ... et al.
Abstract: Neural cell transplantation is an effective way for treatment of neurological diseases. However, the absence of transplantable human neurons remains a barrier for clinical therapies. Human urine-derived cells, namely renal cells and urine stem cells, have become a good source of cells for reprogramming or trans-differentiation research. Here, we show that human urine-derived cells can be partially converted into neuron-like cells by applying a cocktail of small molecules. Gene expression analysis has shown that these induced cells expressed some neuron-specific genes, and a proportion of the cells are GABAergic neurons. Moreover, whole-cell patch clamping recording has shown that some induced cells have neuron-specific voltage gated Na+ and K+ currents but have failed to generate Ca2+ currents and action potentials. Taken together, these results suggest that induced neuronal cells from human urine-derived cells may be useful for neurological disease modelling, drug screening and cell therapies.
Keywords: Induced neuronal cells; Small molecules; Stem cells; Trans-differentiation; Urine-derived cells
Rights: © Springer Nature B.V. 2020.
RMID: 1000017870
DOI: 10.1007/s11033-020-05370-1
Appears in Collections:Medicine publications

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