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|Title:||Conversion of human urine-derived cells into neuron-like cells by small molecules|
|Citation:||Molecular Biology Reports, 2020; 47(4):1-10|
|Donghui Liu, Grigori Rychkov, Mohammed Al‑Hawwas, Nimshitha Pavathuparambil Abdul Manaph, Fiona Zhou, Larisa Bobrovskaya ... et al.|
|Abstract:||Neural cell transplantation is an effective way for treatment of neurological diseases. However, the absence of transplantable human neurons remains a barrier for clinical therapies. Human urine-derived cells, namely renal cells and urine stem cells, have become a good source of cells for reprogramming or trans-differentiation research. Here, we show that human urine-derived cells can be partially converted into neuron-like cells by applying a cocktail of small molecules. Gene expression analysis has shown that these induced cells expressed some neuron-specific genes, and a proportion of the cells are GABAergic neurons. Moreover, whole-cell patch clamping recording has shown that some induced cells have neuron-specific voltage gated Na+ and K+ currents but have failed to generate Ca2+ currents and action potentials. Taken together, these results suggest that induced neuronal cells from human urine-derived cells may be useful for neurological disease modelling, drug screening and cell therapies.|
|Keywords:||Induced neuronal cells; Small molecules; Stem cells; Trans-differentiation; Urine-derived cells|
|Rights:||© Springer Nature B.V. 2020.|
|Appears in Collections:||Medicine publications|
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