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https://hdl.handle.net/2440/124677
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Type: | Journal article |
Title: | Conversion of human urine-derived cells into neuron-like cells by small molecules |
Author: | Liu, D. Rychkov, G. Al-Hawwas, M. Manaph, N.P.A. Zhou, F. Bobrovskaya, L. Liao, H. Zhou, X.-F. |
Citation: | Molecular Biology Reports: an international journal on molecular and cellular biology, 2020; 47(4):1-10 |
Publisher: | Springer |
Issue Date: | 2020 |
ISSN: | 0301-4851 1573-4978 |
Statement of Responsibility: | Donghui Liu, Grigori Rychkov, Mohammed Al‑Hawwas, Nimshitha Pavathuparambil Abdul Manaph, Fiona Zhou, Larisa Bobrovskaya ... et al. |
Abstract: | Neural cell transplantation is an effective way for treatment of neurological diseases. However, the absence of transplantable human neurons remains a barrier for clinical therapies. Human urine-derived cells, namely renal cells and urine stem cells, have become a good source of cells for reprogramming or trans-differentiation research. Here, we show that human urine-derived cells can be partially converted into neuron-like cells by applying a cocktail of small molecules. Gene expression analysis has shown that these induced cells expressed some neuron-specific genes, and a proportion of the cells are GABAergic neurons. Moreover, whole-cell patch clamping recording has shown that some induced cells have neuron-specific voltage gated Na+ and K+ currents but have failed to generate Ca2+ currents and action potentials. Taken together, these results suggest that induced neuronal cells from human urine-derived cells may be useful for neurological disease modelling, drug screening and cell therapies. |
Keywords: | Induced neuronal cells Small molecules Stem cells Trans-differentiation Urine-derived cells |
Rights: | © Springer Nature B.V. 2020. |
DOI: | 10.1007/s11033-020-05370-1 |
Published version: | http://dx.doi.org/10.1007/s11033-020-05370-1 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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