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Type: Journal article
Title: The role of zinc efflux during Acinetobacter baumannii infection
Author: Alquethamy, S.
Adams, F.G.
Naidu, V.
Khorvash, M.
Pederick, V.G.
Zang, M.
Paton, J.C.
Paulsen, I.T.
Hassan, K.
Cain, A.K.
McDevitt, C.A.
Eijkelkamp, B.A.
Citation: ACS Infectious Diseases, 2020; 6(1):150-158
Publisher: American Chemical Society
Issue Date: 2020
ISSN: 2373-8227
Statement of
Saleh F. Alquethamy, Felise G. Adams, Varsha Naidu, Marjan Khorvash, Victoria G. Pederick, Maoge Zang, James C. Paton, Ian T. Paulsen, Karl A. Hassan, Amy K. Cain, Christopher A. McDevitt, and Bart A. Eijkelkamp
Abstract: Acinetobacter baumannii is a highly ubiquitous Gram-negative bacterium, that is associated with significant disease in immunocompromised individuals. The success of A. baumannii is partly attributable to its high level of antibiotic resistance. Further, A. baumannii expresses a broad arsenal of putative zinc efflux systems that are likely to aid environmental persistence and host colonisation, but detailed insights into how the bacterium deals with toxic concentrations of zinc is lacking. In this study we present the transcriptomic responses of A. baumannii to toxic zinc concentrations. Subsequent mutant analyses revealed a primary role for the resistance-nodulation-cell division heavy metal efflux system CzcCBA, and the cation diffusion facilitator transporter CzcD in zinc resistance. To examine the role of zinc at the host-pathogen interface we utilised a murine model of zinc deficiency and challenge with wild-type and czcA mutant cells, which identified highly site-specific roles for zinc during A. baumannii infection. Overall, we provide novel insight into the key zinc resistance mechanisms of A. baumannii and outline the role these systems play in enabling the bacterium to survive in diverse environments.
Keywords: Metal ions; nutrition; infection; transporters; pneumonia
Rights: © 2019 American Chemical Society
RMID: 1000003780
DOI: 10.1021/acsinfecdis.9b00351
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Appears in Collections:Microbiology and Immunology publications

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