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Type: Journal article
Title: High variability in erythrocyte, plasma and whole blood EPA and DHA levels in response to supplementation
Author: Sparkes, C.
Sinclair, A.J.
Gibson, R.A.
Else, P.L.
Meyer, B.J.
Citation: Nutrients, 2020; 12(4)
Publisher: MDPI
Issue Date: 2020
ISSN: 2072-6643
Statement of
Cassandra Sparkes, Andrew J. Sinclair, Robert A. Gibson, Paul L. Else and Barbara J. Meyer
Abstract: (1) Aim: the aim of this secondary analysis was to report the variability in response to n-3 long chain polyunsaturated fatty acids (LCPUFA) supplementation in erythrocytes, plasma and whole blood of a previously published dose response study. (2) Methods: a randomized, double-blind, placebo-controlled trial of parallel design was conducted, whereby pre-menopausal women were randomly assigned to consume 0, 0.35, 0.7 or 1 g/day of supplemental eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA). Fasted blood samples were taken at baseline and after eight weeks intervention. Erythrocyte, plasma and whole blood fatty acids were extracted using the method of Lepage and Roy and analysed using gas chromatography. (3) Results: There were significant increases in EPA plus DHA levels in the 0.7 g and 1 g dose groups, with the highest increase with the 1 g dose notably: in erythrocytes (from 5.69% to 7.59%), plasma (from 2.94% to 5.48%) and in whole blood (from 3.81% to 6.03%). There was high variability in response to the supplement in erythrocytes, plasma and whole blood across the di erent doses. (4) Conclusion: there is high individual variability in n-3 LCPUFA levels in response to n-3 LCPUFA supplementation, which should be taken into account in clinical trials using n-3 LCPUFA supplements.
Keywords: Omega-3; variability; erythrocyte; plasma; whole blood
Rights: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
DOI: 10.3390/nu12041017
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