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Type: Journal article
Title: Phenotypic characterization and comparison of Phe508del and cystic fibrosis transmembrane conductance regulator (CFTR) knockout rat models of cystic fibrosis generated by CRISPR/Cas9 gene editing
Author: McCarron, A.
Cmielewski, P.
Reyne, N.
McIntyre, C.
Finnie, J.
Craig, F.
Rout-Pitt, N.
Delhove, J.
Schjenken, J.E.
Chan, H.Y.
Boog, B.
Knight, E.
Gilmore, R.C.
O'Neal, W.K.
Boucher, R.C.
Parsons, D.
Donnelley, M.
Citation: The American Journal of Pathology, 2020; 190(5):977-993
Publisher: Elsevier
Issue Date: 2020
ISSN: 0002-9440
Statement of
Alexandra McCarron, Patricia Cmielewski, Nicole Reyne, Chantelle McIntyre, John Finnie, Fiona Craig, Nathan Rout-Pitt, Juliette Delhove, John E. Schjenken, Hon Y. Chan, Bernadette Boog, Emma Knight, Rodney C. Gilmore, Wanda K. O' Neal, Richard C. Boucher, David Parsons, and Martin Donnelley
Abstract: Animal models of cystic fibrosis (CF) are essential for investigating disease mechanisms and trialling potential therapeutics. This study generated two CF rat models using clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (Cas9) gene editing. One rat model carries the common human Phe508del (ΔF508) CF transmembrane conductance regulator (CFTR) mutation, whereas the second is a CFTR knockout model. Phenotype was characterized using a range of functional and histological assessments including nasal potential difference to measure electrophysiological function in the upper airways, RNAscope in situ hybridization and quantitative PCR to assess CFTR mRNA expression in the lungs, immunohistochemistry to localize CFTR protein in the airways, and histopathological assessments in a range of tissues. Both rat models revealed a range of CF manifestations including reduced survival, intestinal obstruction, bioelectric defects in the nasal epithelium, histopathological changes in the trachea, large intestine, and pancreas, and abnormalities in the development of the male reproductive tract. The CF rat models presented here will prove useful for longitudinal assessments of pathophysiology and therapeutics.
Keywords: Animals; Mice, Knockout; Humans; Rats; Rats, Sprague-Dawley; Cystic Fibrosis; Disease Models, Animal; Cystic Fibrosis Transmembrane Conductance Regulator; Phenotype; Mutation; CRISPR-Cas Systems; Gene Editing
Rights: Copyright © 2020 American Society for Investigative Pathology.
RMID: 1000014924
DOI: 10.1016/j.ajpath.2020.01.009
Grant ID:
Appears in Collections:Medicine publications

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