Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/124888
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Type: Journal article
Title: Bacopasides I and II act in synergy to inhibit the growth, migration and invasion of breast cancer cell lines
Author: Palethorpe, H.M.
Smith, E.
Tomita, Y.
Nakhjavani, M.
Yool, A.J.
Price, T.J.
Young, J.P.
Townsend, A.R.
Hardingham, J.E.
Citation: Molecules, 2019; 24(19):3539-1-3539-16
Publisher: MDPI AG
Issue Date: 2019
ISSN: 1420-3049
1420-3049
Statement of
Responsibility: 
Helen M. Palethorpe, Eric Smith, Yoko Tomita, Maryam Nakhjavani, Andrea J. Yool, Timothy J. Price, Joanne P. Young, Amanda R. Townsend and Jennifer E. Hardingham
Abstract: Bacopaside (bac) I and II are triterpene saponins purified from the medicinal herb Bacopa monnieri. Previously, we showed that bac II reduced endothelial cell migration and tube formation and induced apoptosis in colorectal cancer cell lines. The aim of the current study was to examine the effects of treatment with combined doses of bac I and bac II using four cell lines representative of the breast cancer subtypes: triple negative (MDA-MB-231), estrogen receptor positive (T47D and MCF7) and human epidermal growth factor receptor 2 (HER2) positive (BT-474). Drug treatment outcome measures included cell viability, proliferation, cell cycle, apoptosis, migration, and invasion assays. Relationships were analysed by one- and two-way analysis of variance with Bonferroni post-hoc analysis. Combined doses of bac I and bac II, each below their half maximal inhibitory concentration (IC50), were synergistic and reduced the viability and proliferation of the four breast cancer cell lines. Cell loss occurred at the highest dose combinations and was associated with G2/M arrest and apoptosis. Migration in the scratch wound assay was significantly reduced at apoptosis-inducing combinations, but also at non-cytotoxic combinations, for MDA-MB-231 and T47D (p < 0.0001) and BT-474 (p = 0.0003). Non-cytotoxic combinations also significantly reduced spheroid invasion of MDA-MB-231 cells by up to 97% (p < 0.0001). Combining bac I and II below their IC50 reduced the viability, proliferation, and migration and invasiveness of breast cancer cell lines, suggesting synergy between bac I and II.
Keywords: bacopaside I; bacopaside II; synergy; breast cancer; migration; spheroid invasion; triple negative breast cancer
Description: Published: 30 September 2019
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
RMID: 1000001335
DOI: 10.3390/molecules24193539
Appears in Collections:Medicine publications

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