Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/124965
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Type: Journal article
Title: Measuring metabolic rates of small terrestrial organisms by fluorescence-based closed-system respirometry
Author: Tomlinson, S.
Dalziell, E.L.
Withers, P.C.
Lewandrowski, W.
Dixon, K.W.
Merritt, D.J.
Citation: The Journal of Experimental Biology, 2018; 221(7):jeb172874-1-jeb172874-8
Publisher: Company of Biologists
Issue Date: 2018
ISSN: 0022-0949
1477-9145
Statement of
Responsibility: 
Sean Tomlinson, Emma L. Dalziell, Philip C. Withers, Wolfgang Lewandrowski, Kingsley W. Dixon, and David J. Merritt
Abstract: We explore a recent, innovative variation of closed-system respirometry for terrestrial organisms, whereby oxygen partial pressure (PO₂ ) is repeatedly measured fluorometrically in a constant-volume chamber over multiple time points. We outline a protocol that aligns this technology with the broader literature on aerial respirometry, including the calculations required to accurately convert O₂ depletion to metabolic rate (MR). We identify a series of assumptions, and sources of error associated with this technique, including thresholds where O₂ depletion becomes limiting, that impart errors to the calculation and interpretation of MR. Using these adjusted calculations, we found that the resting MR of five species of angiosperm seeds ranged from 0.011 to 0.640 ml g⁻¹ h⁻¹, consistent with published seed MR values. This innovative methodology greatly expands the lower size limit of terrestrial organisms that can be measured, and offers the potential for measuring MR changes over time as a result of physiological processes of the organism.
Keywords: Respirometer; constant volume; Q₂ technology; oxygen consumption; metabolism; seed biology
Rights: © 2018. Published by The Company of Biologists Ltd
DOI: 10.1242/jeb.172874
Grant ID: http://purl.org/au-research/grants/arc/LP160100381
http://purl.org/au-research/grants/arc/IC150100041
Appears in Collections:Aurora harvest 4
Ecology, Evolution and Landscape Science publications

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