Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/124967
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments
Author: Zhang, J.
Czabotar, P.
Policheni, A.
Caminschi, I.
San Wan, S.
Kitsoulis, S.
Tullett, K.
Robin, A.
Brammananth, R.
van Delft, M.
Lu, J.
O'Reilly, L.
Josefsson, E.
Kile, B.
Chin, W.
Mintern, J.
Olshina, M.
Wong, W.
Baum, J.
Wright, M.
et al.
Citation: Immunity, 2012; 36(4):646-657
Publisher: CellL Press
Issue Date: 2012
ISSN: 1074-7613
1097-4180
Statement of
Responsibility: 
Jian-Guo Zhang, Peter E. Czabotar, Antonia N. Policheni, Nicos A. Nicola, Ken Shortman, Mireille H. Lahoud
Abstract: The immune system must distinguish viable cells from cells damaged by physical and infective processes. The damaged cell-recognition molecule Clec9A is expressed on the surface of the mouse and human dendritic cell subsets specialized for the uptake and processing of material from dead cells. Clec9A recognizes a conserved component within nucleated and nonnucleated cells, exposed when cell membranes are damaged. We have identified this Clec9A ligand as a filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins. We have determined the crystal structure of the human CLEC9A C-type lectin domain and propose a functional dimeric structure with conserved tryptophans in the ligand recognition site. Mutation of these residues ablated CLEC9A binding to damaged cells and to the isolated ligand complexes. We propose that Clec9A provides targeted recruitment of the adaptive immune system during infection and can also be utilized to enhance immune responses generated by vaccines.
Keywords: Dendritic Cells
Rights: ©2012 Elsevier Inc.
RMID: 1000001884
DOI: 10.1016/j.immuni.2012.03.009
Grant ID: http://purl.org/au-research/grants/nhmrc/454465
http://purl.org/au-research/grants/nhmrc/461219
http://purl.org/au-research/grants/nhmrc/461221
http://purl.org/au-research/grants/nhmrc/575546
http://purl.org/au-research/grants/nhmrc/1009145
Appears in Collections:Environment Institute publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.