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Type: Journal article
Title: Effects of β-hydroxybutyricacid on the synthesis and assembly of very low-density lipoprotein in bovine hepatocytes in vitro
Other Titles: Effects of beta-hydroxybutyricacid on the synthesis and assembly of very low-density lipoprotein in bovine hepatocytes in vitro
Author: Deng, Q.
Ma, D.
Shi, Z.
Huang, W.
Du, X.
Gao, W.
Zhu, X.
Lei, L.
Zhang, M.
Sun, G.
Yuan, X.
Li, X.
Wang, Z.
Liu, G.
Li, X.
Citation: Journal of Animal Physiology and Animal Nutrition, 2016; 100(2):331-336
Publisher: Wiley Online Library
Issue Date: 2016
ISSN: 0931-2439
Statement of
Q. Deng, D. Ma, Z. Shi, W. Huang, X. Du, W. Gao ... et al.
Abstract: β-Hydroxybutyricacid (BHBA) is an important metabolite that involved in the development of ketosis and fatty liver in dairy cows. Dairy cows with fatty liver displayed high blood concentration of BHBA and very low-density lipoprotein (VLDL) assembly. The effects of BHBA on VLDL synthesis and assembly in hepatocytes of cows were unclear. In this study, bovine hepatocytes were cultured and treated with different concentrations of BHBA. We found that BHBA treatment upregulated the mRNA and protein levels of apolipoprotein B100 (ApoB 100), apolipoprotein E (ApoE) and microsomal triglyceride transfer protein (MTTP) and showed in a firstly increased and then decreased trend. Meanwhile, the mRNA and protein levels of LDLR showed in a reverse trend. Consequently, VLDL content was significantly increased in medium-dose BHBA treatment group, while decreased in high-dose group. These results indicate that the effects of BHBA on the VLDL synthesis showed in a dose-dependent manner that low levels of BHBA increase VLDL synthesis and high levels of BHBA decrease VLDL synthesis.
Keywords: β ‐Hydroxybutyricacid; bovine hepatocyte; very low‐density lipoprotein; negative energy balance; synthesis and assembly
Rights: Journal of Animal Physiology and Animal Nutrition © 2015 Blackwell Verlag GmbH
RMID: 0030084562
DOI: 10.1111/jpn.12380
Appears in Collections:Medicine publications

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