Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/126093
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Type: Journal article
Title: The formation of a functional retinal pigment epithelium occurs on porous polytetrafluoroethylene substrates independently of the surface chemistry
Author: Kearns, V.
Tasker, J.
Zhuola
Akhtar, R.
Bachhuka, A.
Vasilev, K.
Sheridan, C.
Williams, R.
Citation: Journal of Materials Science: Materials in Medicine, 2017; 28(8):124-1-124-14
Publisher: Springer Nature
Issue Date: 2017
ISSN: 0957-4530
1573-4838
Statement of
Responsibility: 
Victoria R. Kearns, Jack Tasker, Zhuola, Riaz Akhtar, Akash Bachhuka, Krasimir Vasilev, Carl M. Sheridan, Rachel L. Williams
Abstract: Subretinal transplantation of functioning retinal pigment epithelial (RPE) cells may have the potential to preserve or restore vision in patients affected by blinding diseases such as age-related macular degeneration (AMD). One of the critical steps in achieving this is the ability to grow a functioning retinal pigment epithelium, which may need a substrate on which to grow and to aid transplantation. Tailoring the physical and chemical properties of the substrate should help the engineered tissue to function in the long term. The purpose of the study was to determine whether a functioning monolayer of RPE cells could be produced on expanded polytetrafluoroethylene substrates modified by either an ammonia plasma treatment or an n-Heptylamine coating, and whether the difference in surface chemistries altered the extracellular matrix the cells produced. Primary human RPE cells were able to form a functional, cobblestone monolayer on both substrates, but the formation of an extracellular matrix to exhibit a network structure took months, whereas on non-porous substrates with the same surface chemistry, a similar appearance was observed after a few weeks. This study suggests that the surface chemistry of these materials may not be the most critical factor in the development of growth of a functional monolayer of RPE cells as long as the cells can attach and proliferate on the surface. This has important implications in the design of strategies to optimise the clinical outcomes of subretinal transplant procedures.
Keywords: Cells, Cultured
Extracellular Matrix
Humans
Polytetrafluoroethylene
Tissue Culture Techniques
Tissue Engineering
Materials Testing
Cell Communication
Cell Proliferation
Surface Properties
Porosity
Tissue Scaffolds
Retinal Pigment Epithelium
Primary Cell Culture
Rights: © The Author(s) 2017. This article is an open access publication. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
DOI: 10.1007/s10856-017-5926-3
Appears in Collections:Aurora harvest 8
Molecular and Biomedical Science publications

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