Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/126221
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Type: Journal article
Title: Expression of cholesterol packaging and transport genes in human and rat placenta: impact of obesity and a high-fat diet
Author: Draycott, S.A.V.
Daniel, Z.
Khan, R.
Muhlhausler, B.S.
Elmes, M.J.
Langley-Evans, S.C.
Citation: Journal of Developmental Origins of Health and Disease, 2020; 11(3):222-227
Publisher: Cambridge University Press
Issue Date: 2020
ISSN: 2040-1744
2040-1752
Statement of
Responsibility: 
Sally A.V. Draycott, Zoe Daniel, Raheela Khan, Beverly S. Muhlhausler, Matthew J. Elmes, and Simon C. Langley-Evans
Abstract: Evidence suggests that sub-optimal maternal nutrition has implications for the developing offspring. We have previously shown that exposure to a low-protein diet during gestation was associated with upregulation of genes associated with cholesterol transport and packaging within the placenta. This study aimed to elucidate the effect of altering maternal dietary linoleic acid (LA; omega-6) to alpha-linolenic acid (ALA; omega-6) ratios as well as total fat content on placental expression of genes associated with cholesterol transport. The potential for maternal body mass index (BMI) to be associated with expression of these genes in human placental samples was also evaluated. Placentas were collected from 24 Wistar rats at 20-day gestation (term = 21-22-day gestation) that had been fed one of four diets containing varying fatty acid compositions during pregnancy, and from 62 women at the time of delivery. Expression of 14 placental genes associated with cholesterol packaging and transfer was assessed in rodent and human samples by quantitative real time polymerase chain reaction. In rats, placental mRNA expression of ApoA2, ApoC2, Cubn, Fgg, Mttp and Ttr was significantly elevated (3-30 fold) in animals fed a high LA (36% fat) diet, suggesting increased cholesterol transport across the placenta in this group. In women, maternal BMI was associated with fewer inconsistent alterations in gene expression. In summary, sub-optimal maternal nutrition is associated with alterations in the expression of genes associated with cholesterol transport in a rat model. This may contribute to altered fetal development and potentially programme disease risk in later life. Further investigation of human placenta in response to specific dietary interventions is required.
Keywords: Cholesterol; maternal nutrition; omega; placenta; pregnancy
Rights: © Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2019
DOI: 10.1017/S2040174419000606
Grant ID: http://purl.org/au-research/grants/nhmrc/1083009
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