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Type: Journal article
Title: A genomic perspective of metal-resistant bacteria from gold particles: Possible survival mechanisms during gold biogeochemical cycling
Author: Sanyal, S.K.
Reith, F.
Shuster, J.
Citation: FEMS Microbiology Ecology, 2020; 96(7):fiaa111-1-fiaa111-15
Publisher: Oxford University Press (OUP)
Issue Date: 2020
ISSN: 0168-6496
Statement of
Santonu Kumar Sanyal, Frank Reith and Jeremiah Shuster
Abstract: A bacterial consortium was enriched from gold particles that 'experienced' ca. 80 years of biotransformation within waste-rock piles (Australia). This bacterial consortium was exposed to 10 µM AuCl3 to obtain Au-tolerant bacteria. From these isolates, Serratia sp. and Stenotrophomonas sp. were the most Au-tolerant and reduced soluble Au as pure gold nanoparticles, indicating that passive mineralisation is a mechanism for mediating the toxic effect of soluble Au produced during particle dissolution. Genome-wide analysis demonstrated that these isolates also possessed various genes that could provide cellular defence enabling survival under heavy-metal stressed condition by mediating the toxicity of heavy metals through active efflux/reduction. Diverse metal-resistant genes or genes clusters (cop, cus, czc, znt, ars) were detected, which could confer resistance to soluble Au. Comparative genome analysis revealed that the majority of detected heavy-metal resistant genes were similar (i.e. orthologous) to those genes of Cupriavidus metallidurans CH34. The detection of heavy-metal resistance, nutrient cycling, and biofilm formation genes (pgaABCD, bsmA, hmpS) may have indirect yet important roles when dealing with soluble Au during particle dissolution. In conclusion, the physiological and genomic results suggest that bacteria living on gold particles would likely use various genes to ensure survival during Au biogeochemical cycling.
Keywords: Gold particles; Heavy-metal resistance; Serratia; Stenotrophomonas; Gold biogeochemistry; Gold biogeochemical cycling; Au-tolerant bacteria
Description: Advance Access Publication Date: 4 June 2020.
Rights: © FEMS 2020. All rights reserved.
RMID: 1000021549
DOI: 10.1093/femsec/fiaa111
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Appears in Collections:Microbiology and Immunology publications

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