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Type: Journal article
Title: Bright fluorescent vacuolar marker lines allow vacuolar tracing across multiple tissues and stress conditions in rice
Author: Cao, Y.
Cai, W.
Chen, X.
Chen, M.
Chu, J.
Liang, W.
Persson, S.
Liu, Z.
Zhang, D.
Citation: International Journal of Molecular Sciences, 2020; 21(12):4203-1-4203-16
Publisher: MDPI
Issue Date: 2020
ISSN: 1661-6596
Statement of
Yiran Cao, Wenguo Cai, Xiaofei Chen, Mingjiao Chen, Jianjun Chu, Wanqi Liang, Staffan Persson, Zengyu Liu, and Dabing Zhang
Abstract: The vacuole is indispensable for cells to maintain their water potential and to respond to environmental changes. Nevertheless, investigations of vacuole morphology and its functions have been limited to Arabidopsis thaliana with few studies in the model crop rice (Oryza sativa). Here, we report the establishment of bright rice vacuole fluorescent reporter systems using OsTIP1;1, a tonoplast water channel protein, fused to either an enhanced green fluorescent protein or an mCherry red fluorescent protein. We used the corresponding transgenic rice lines to trace the vacuole morphology in roots, leaves, anthers, and pollen grains. Notably, we observed dynamic changes in vacuole morphologies in pollen and root epidermis that corresponded to their developmental states as well as vacuole shape alterations in response to abiotic stresses. Our results indicate that the application of our vacuole markers may aid in understanding rice vacuole function and structure across different tissues and environmental conditions in rice.
Keywords: Oryza sativa; vacuole; fluorescent marker; tonoplast intrinsic protein
Rights: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
DOI: 10.3390/ijms21124203
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Appears in Collections:Agriculture, Food and Wine publications
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