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Type: Journal article
Title: A novel rat model to test intra-abdominal anti-adhesive therapy
Author: Vediappan, R.S.
Bennett, C.
Bassiouni, A.
Smith, M.
Finnie, J.
Trochsler, M.
Psaltis, A.J.
Vreugde, S.
Wormald, P.J.
Citation: Frontiers in Surgery, 2020; 7:12-12
Publisher: Frontiers
Issue Date: 2020
ISSN: 2296-875X
Statement of
Rajan Sundaresan Vediappan, Catherine Bennett, Ahmed Bassiouni, Matthew Smith, John Finnie, Markus Trochsler ... et al.
Abstract: Background: Adhesion formation after abdominal surgery is considered almost inevitable and a major cause of morbidity. Novel treatments have been proposed, however there is a lack of suitable small animal models for pre-clinical evaluation, mainly due to inconsistency in adhesion formation in positive control animals. Here, we propose a new rat model of abdominal adhesions using Kaolin as the adhesion-inducing agent at an optimized dosage for testing newer agents in respect to their anti-adhesive property. Materials and Methods: Twenty-five adult (8-10 week old) male Wistar albino rats underwent midline laparotomy and caecal abrasion and were randomized to receive topical applications of normal saline or different concentrations and volumes of a Kaolin-based formulation. At day 14 rats were humanely killed, and adhesions graded macroscopically by an investigator blinded to the treatment groups, using pre-determined adhesion scores and microscopically using histopathology. Results: Kaolin at 0.005 g/mL caused consistent adhesions without compromising rat viability. At higher doses significant morbidity and mortality was observed in the animals treated. Conclusions: Kaolin induced adhesion in a rat abdominal surgery model is reliable and can be safely used to test the efficacy of novel anti-adhesive formulations to prevent intra-abdominal adhesions.
Keywords: Kaolin
abdominal adhesion
animal model
anti-adhesive agent
Rights: © 2020 Vediappan, Bennett, Bassiouni, Smith, Finnie, Trochsler, Psaltis, Vreugde and Wormald. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI: 10.3389/fsurg.2020.00012
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