Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/127404
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Type: Journal article
Title: Proteomic analysis of plasmodesmata from Populus cell suspension cultures in relation with callose biosynthesis
Author: Leijon, F.
Melzer, M.
Zhou, Q.
Srivastava, V.
Bulone, V.
Citation: Frontiers in Plant Science, 2018; 9:1681-1-1681-14
Publisher: Frontiers Media
Issue Date: 2018
ISSN: 1664-462X
1664-462X
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Responsibility: 
Felicia Leijon, Michael Melzer, Qi Zhou, Vaibhav Srivastava and Vincent Bulone
Abstract: Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signaling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well-defined. Here, we have isolated fractions enriched in plasmodesmata from cell suspension cultures of Populus trichocarpa and identified 201 proteins that are enriched in these fractions, thereby providing further insight on the multiple functions of plasmodesmata. Proteomics analysis revealed an enrichment of proteins specifically involved in responses to stress, transport, metabolism and signal transduction. Consistent with the role of callose deposition and turnover in the closure and aperture of the plasmodesmata and our proteomic analysis, we demonstrate the enrichment of callose synthase activity in the plasmodesmata represented by several gene products. A new form of calcium-independent callose synthase activity was detected, in addition to the typical calcium-dependent enzyme activity, suggesting a role of calcium in the regulation of plasmodesmata through two forms of callose synthase activities. Our report provides the first proteomic investigation of the plasmodesmata from a tree species and the direct biochemical evidence for the occurrence of several forms of active callose synthases in these structures. Data are available via ProteomeXchange with identifier PXD010692.
Keywords: Populus; plasmodesmata; callose synthase; callose; mass spectrometry; spectral counting
Rights: © 2018 Leijon, Melzer, Zhou, Srivastava and Bulone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
DOI: 10.3389/fpls.2018.01681
Appears in Collections:Agriculture, Food and Wine publications
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