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Type: Journal article
Title: Racemization at the Asp 58 residue in αA-crystallin from the lens of high myopic cataract patients
Other Titles: Racemization at the Asp 58 residue in alphaA-crystallin from the lens of high myopic cataract patients
Author: Zhu, X.
Zhang, K.
He, W.
Du, Y.
Hooi, M.
Lu, Y.
Citation: Journal of Cellular and Molecular Medicine, 2018; 22(2):1118-1126
Publisher: Wiley
Issue Date: 2018
ISSN: 1582-1838
Statement of
Xiang-jia Zhu, Ke-ke Zhang, Wen-wen He, Yu Du, Michelle Hooi, Yi Lu
Abstract: Post-translational modifications in lens proteins are key causal factors in cataract. As the most abundant post-translational modification in the lens, racemization may be closely related to the pathogenesis of cataract. Racemization of αA-crystallin, a crucial structural and heat shock protein in the human lens, could significantly influence its structure and function. In previous studies, elevated racemization from l-Asp 58 to d-isoAsp58 in αA-crystallin has been found in age-related cataract (ARC) lenses compared to normal aged human lenses. However, the role of racemization in high myopic cataract (HMC), which is characterized by an early onset of nuclear cataract, remains unknown. In the current study, apparently different from ARC, significantly increased racemization from l-Asp 58 to d-Asp 58 in αA-crystallin was identified in HMC lenses. The average racemization rates for each Asp isoform were calculated in ARC and HMC group. In ARC patients, the conversion of l-Asp 58 to d-isoAsp 58, up to 31.89%, accounted for the main proportion in racemization, which was in accordance with the previous studies. However, in HMC lenses, the conversion of l-Asp 58 to d-Asp 58, as high as 35.44%, accounted for the largest proportion of racemization in αA-crystallin. The different trend in the conversion of αA-crystallin by racemization, especially the elevated level of d-Asp 58 in HMC lenses, might prompt early cataractogenesis and a possible explanation of distinct phenotypes of cataract in HMC.
Keywords: Aspartyl residue
high myopia
Rights: © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1111/jcmm.13363
Appears in Collections:Aurora harvest 4
Molecular and Biomedical Science publications

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