Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/127417
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dc.contributor.authorKamran, M.en
dc.contributor.authorRamesh, S.A.en
dc.contributor.authorGilliham, M.en
dc.contributor.authorTyerman, S.D.en
dc.contributor.authorBose, J.en
dc.date.issued2020en
dc.identifier.citationPlant, Cell and Environment, 2020; 43(10):2443-2459en
dc.identifier.issn0140-7791en
dc.identifier.issn1365-3040en
dc.identifier.urihttp://hdl.handle.net/2440/127417-
dc.description.abstractMalate exudation through wheat (Triticum aestivum L) Aluminium-activated Malate Transporter 1 (TaALMT1) confers Al³⁺ tolerance at low pH, but is also activated by alkaline pH, and is regulated by and facilitates significant transport of gamma-aminobutyric acid (GABA, a zwitterionic buffer). Therefore, TaALMT1 may facilitate acidification of an alkaline rhizosphere by promoting exudation of both malate and GABA. Here, the performance of wheat near isogenic lines (NILs) ET8 (Al⁺³ -tolerant, high TaALMT1 expression) and ES8 (Al⁺³ -sensitive, low TaALMT1 expression) are compared. Root growth (at 5-weeks) was higher for ET8 than ES8 at pH 9. ET8 roots exuded more malate and GABA at high pH and acidified the rhizosphere more rapidly. GABA and malate exudation was enhanced at high pH by the addition of aluminate in both ET8 and transgenic barley expressing TaALMT1. Xenopus laevis oocytes expressing TaALMT1 acidified an alkaline media more rapidly than controls corresponding to higher GABA efflux. TaALMT1 expression did not change under alkaline conditions but key genes involved in GABA turnover changed in accord with a high rate of GABA synthesis. We propose that TaALMT1 plays a role in alkaline tolerance by exuding malate and GABA, possibly coupled to proton efflux.en
dc.description.statementofresponsibilityMuhammad Kamran, Sunita A. Ramesh, Matthew Gilliham, Stephen D. Tyerman, Jayakumar Boseen
dc.language.isoenen
dc.publisherWileyen
dc.rights© 2020 John Wiley & Sons Ltden
dc.subjectAluminate; aluminum; barley; gama-aminobutyric acid; Hordeum vulgare; proton efflux; rhizosphere acidification; Triticum aestivumen
dc.titleRole of TaALMT1 malate-GABA transporter in alkaline pH tolerance of wheaten
dc.typeJournal articleen
dc.identifier.rmid1000023276en
dc.identifier.doi10.1111/pce.13845en
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100008en
dc.relation.granthttp://purl.org/au-research/grants/arc/DE170100346en
dc.identifier.pubid538274-
pubs.library.collectionAgriculture, Food and Wine publicationsen
pubs.library.teamDS14en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidRamesh, S.A. [0000-0003-2230-4737]en
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]en
dc.identifier.orcidTyerman, S.D. [0000-0003-2455-1643]en
dc.identifier.orcidBose, J. [0000-0002-0565-2951]en
Appears in Collections:Agriculture, Food and Wine publications

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