Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/127525
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dc.contributor.advisorElliott, W.H.-
dc.contributor.authorParslow, Graham Royston-
dc.date.issued1978-
dc.identifier.urihttp://hdl.handle.net/2440/127525-
dc.description.abstract1. A Sample of rat liver mitochondrial ALV-Synthetase was purified to a specific activity of 4,684 units/ mg, the highest activity yet observed from a mammalian source. 2. The sequence of purification steps that permitted the isolation of the high specific activity enzyme noted above was developed during the work reported here. The sequence of procedures finally used to purify a mitochondrial extract included 0-50% ammonium sulphate precipitation, 5-20% (w/v) polyethylene glycol precipation, CM-sephadex chromatography, Sephadex G-100 filtration, and electrophoresis. 3. Attempts to duplicate previously reported purifications of ALV-Synthetase by use of affinity chromatography were unsuccessful. 4. Isoelectric focusing gave no clearly useful or preparative separations of ALV-Synthetase in pH gradients.en
dc.language.isoenen
dc.titleInvestigation of purification procedures to isolate rat mitochondrial ẟ - aminolaevulinic acid synthetaseen
dc.typeThesisen
dc.contributor.schoolSchool of Biological Sciencesen
dc.provenanceThis electronic version is made publicly available by the University of Adelaide in accordance with its open access policy for student theses. Copyright in this thesis remains with the author. This thesis may incorporate third party material which has been used by the author pursuant to Fair Dealing exceptions. If you are the owner of any included third party copyright material you wish to be removed from this electronic version, please complete the take down form located at: http://www.adelaide.edu.au/legalsen
dc.description.dissertationThesis (M.Sc.) -- University of Adelaide, Dept. of Biochemistry, 1979en
Appears in Collections:Research Theses

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