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|Title:||Identification of DNA response elements regulating expression of CCAAT/enhancer-binding protein (C/EBP) β and δ and MAP kinase-interacting kinases during early adipogenesis|
|Other Titles:||Identification of DNA response elements regulating expression of CCAAT/enhancer-binding protein (C/EBP) beta and delta and MAP kinase-interacting kinases during early adipogenesis|
|Citation:||Adipocyte, 2020; 9(1):427-442|
|James E. Merrett , Tao Bo , Peter J. Psaltis and Christopher G. Proud|
|Abstract:||Given the high and increasing prevalence of obesity and associated disorders, such as type-2 diabetes, it is important to understand the mechanisms that regulate lipid storage and the differentiation of fat cells, a process termed adipogenesis. Using the well-established mouse 3T3-L1 in vitro model of adipogenesis, we refine how the induction of two key adipogenic transcription factors, CCAAT/enhancer-binding proteins (C/EBPs) β and δ are regulated during early adipogenesis. We identify, in the gene promoters of Cebpb and Cebpd, the DNA response elements responsible for binding transcription factors that are activated by cAMP or glucocorticoids. We also show that mitogen-activated protein kinase (MAPK)-interacting kinase 2 (MNK2; Mknk2), which plays a distinct role in diet-induced obesity, is induced during early adipogenesis and identify the functional DNA response elements responsible for regulating its expression. Mknk2 expression is maintained in differentiated 3T3-L1 adipocytes and is expressed at high levels across a range of mouse adipose tissue depots. Together, these new insights help to clarify the transcriptional programme of early adipogenesis and identify Mknk2 as one of potentially many genes up-regulated during adipogenesis.|
|Keywords:||Adipogenesis; CCAAT/enhancer‐binding protein (C/EBP); MAPK-interacting kinase (MNK); adipocyte; cAMP response element‐binding protein (CREB); cyclic AMP (cAMP); glucocorticoid; glucocorticoid receptor|
|Rights:||© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.|
|Appears in Collections:||Environment Institute publications|
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