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Type: Journal article
Title: Trichoderma harzianum inoculation reduces the incidence of clubroot disease in Chinese cabbage by regulating the rhizosphere microbial community
Author: Li, J.
Philp, J.
Li, J.
Wei, Y.
Li, H.
Yang, K.
Ryder, M.
Toh, R.
Zhou, Y.
Denton, M.D.
Hu, J.
Wang, Y.
Citation: Microorganisms, 2020; 8(9):1325-1-1325-17
Publisher: MDPI
Issue Date: 2020
ISSN: 2076-2607
Statement of
Junhui Li, Joshua Philp, Jishun Li, Yanli Wei, Hongmei Li, Kai Yang, Maarten Ryder, Ruey Toh, Yi Zhou, Matthew D. Denton, Jindong Hu, and Yan Wang
Abstract: Clubroot is a disease of cruciferous crops that causes significant economic losses to vegetable production worldwide. We applied high-throughput amplicon sequencing technology to quantify the effect of Trichodermaharzianum LTR-2 inoculation on the rhizosphere community of Chinese cabbage (Brassica rapa subsp. pekinensis cv. Jiaozhou) in a commercial production area. T. harzianum inoculation of cabbage reduced the incidence of clubroot disease by 45.4% (p < 0.05). The disease control efficacy (PDIDS) was 63%. This reduction in disease incidence and severity coincided with a drastic reduction in both the relative abundance of Plasmodiaphora brassicae, the causative pathogen of cabbage clubroot disease, and its copy number in rhizosphere soil. Pathogenic fungi Alternaria and Fusarium were also negatively associated with Trichoderma inoculation according to co-occurrence network analysis. Inoculation drastically reduced the relative abundance of the dominant bacterial genera Delftia and Pseudomonas, whilst increasing others including Bacillus. Our results demonstrate that T. harzianum LTR-2 is an effective biological control agent for cabbage clubroot, which acts through modulation of the soil and rhizosphere microbial community.
Keywords: Clubroot; high-throughput amplicon; Trichoderma harzianum; Plasmodiaphora brassicae; microbial community
Rights: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
DOI: 10.3390/microorganisms8091325
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Appears in Collections:Agriculture, Food and Wine publications
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