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Type: Journal article
Title: A novel method for detecting antigen-specific human regulatory T cells
Author: Ebert, L.
MacRaild, S.
Davis, I.
Cebon, J.
Chen, W.
Citation: Journal of Immunological Methods, 2012; 377(1-2):56-61
Publisher: Elsevier
Issue Date: 2012
ISSN: 0022-1759
Statement of
Lisa M.Ebert, Sarah E.MacRaild, Ian D.Davis, Jonathan Cebon, Weisan Chen
Abstract: Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.
Keywords: Fox P3, Antigen-specific regulatory T cells; CD3 down-regulation; NY-ESO-1
Rights: © 2012 Elsevier B.V. All rights reserved.
DOI: 10.1016/j.jim.2012.01.004
Appears in Collections:Aurora harvest 8
Biochemistry publications

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