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|Title:||A novel method for detecting antigen-specific human regulatory T cells|
|Citation:||Journal of Immunological Methods, 2012; 377(1-2):56-61|
|Lisa M.Ebert, Sarah E.MacRaild, Ian D.Davis, Jonathan Cebon, Weisan Chen|
|Abstract:||Antigenic epitopes recognized by FoxP3(+) regulatory T cells (Treg) are poorly defined, largely due to a lack of assays for determining Treg specificity. We have developed a novel approach for detecting human Treg specific to peptide antigen, utilizing down-regulation of surface CD3 as a read-out of antigen recognition. Culture conditions and re-stimulation time have been optimized, allowing the detection of even very rare Treg, such as those specific to tumor antigens.|
|Keywords:||Fox P3, Antigen-specific regulatory T cells; CD3 down-regulation; NY-ESO-1|
|Rights:||© 2012 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Biochemistry publications|
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