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|Title:||Shigella flexneri targets human colonic goblet cells by O antigen binding to Sialyl-Tn and Tn antigens via glycan-glycan interactions|
|Citation:||ACS Infectious Diseases, 2020; 6(10):2604-2615|
|Publisher:||American Chemical Society|
|Elizabeth Ngoc Hoa Tran, Christopher J. Day, Erin McCartney, Jessica Poole, Edmund Tse, Michael P. Jennings, and Renato Morona|
|Abstract:||Shigella flexneri targets colonic cells in humans to initiate invasive infection processes that lead to dysentery, and direct interactions between their lipopolysaccharide O antigens and blood group A related glycans are involved in the cell adherence interactions. Here, we show that treatment with Tn and sialyl-Tn glycans, monoclonal antibodies and lectins reactive to Tn/sialyl-Tn, and luteolin (a Tn antigen synthesis inhibitor) all significantly inhibited S. flexneri adherence and invasion of cells in vitro. Surface plasmon resonance analysis showed that lipopolysaccharide O antigen had a high affinity interaction with Tn/sialyl-Tn. Immunofluorescence probing of human colon tissue with antibodies detected expression of Tn/sialyl-Tn by MUC2 producing goblet cells (GCs), and S. flexneri incubated with human colon tissue colocalized with GCs. Our findings demonstrate that S. flexneri targets GCs in the human colonic crypts via glycan-glycan interactions, establishing new insight into the infection process in humans.|
|Keywords:||Shigella flexneri; Tn glycan; sialyl-Tn glycan; colonic crypts; goblet cells; glycan−glycan|
|Rights:||© 2020 American Chemical Society|
|Appears in Collections:||Molecular and Biomedical Science publications|
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