Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/129514
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Type: Journal article
Title: Physical mapping of chromosome 7J and a purple coleoptile gene from Thinopyrum intermedium in the common wheat background
Author: Li, J.
Yu, Z.
Zhang, A.
Yin, Y.
Tang, L.
Li, G.
Zhang, P.
Khan, I.A.
Dundas, I.
Yang, Z.
Citation: Planta: an international journal of plant biology, 2021; 253(1):22-1-22-12
Publisher: Springer Science and Business Media LLC
Issue Date: 2021
ISSN: 0032-0935
1432-2048
Statement of
Responsibility: 
Jianbo Li, Zhihui Yu, Ahui Zhang, Yan Yin, Lingrong Tang, Guangrong Li, Peng Zhang, Imtiaz Ahmed Khan, Ian Dundas, Zujun Yang
Abstract: Main conclusion: A physical map of Thinopyrum intermedium chromosome 7J was constructed using translocation mapping, and a new seedling purple coleoptile gene was mapped to the bin of FL 0.35-0.63 of 7JS. Abstract: Thinopyrum intermedium (2n = 6x = 42, JJJsJsStSt), a wild relative of common wheat, harbors numerous beneficial genes for wheat improvement. Previous studies showed that wheat-Th. intermedium partial amphiploid TAF46 and its derived addition line L1 had a purple coleoptile, which was derived from Th. intermedium chromosome 7J. To identify and physically map the purple coleoptile gene, 12 wheat-Th. intermedium 7J translocation lines were analyzed by sequential multicolor fluorescence in situ hybridization (mc-FISH), PCR-based landmark unique gene (PLUG) and intron targeting (IT) markers. A physical map of the 7J chromosome was constructed, consisting of eight chromosomal bins with 89 markers. Seedling evaluation of the coleoptile colors of all tested materials indicated that the purple coleoptile gene was located to the bin with a fraction length (FL) of 0.35-0.63 on chromosome 7JS. Furthermore, based on the syntenic relationships between Th. intermedium and wheat chromosomes, we developed a new chromosome 7J-specific EST-PCR marker from the chromosomal region corresponding to the purple coleoptile gene through the Triticeae multi-omics database. The approach of designing chromosome-specific markers has facilitated fine mapping of the Thinopyrum-specific purple coleoptile gene, and these translocation lines will be valuable for studying the function of the purple coleoptile gene in anthocyanin biosynthesis.
Keywords: Chromosomes, Plant
Poaceae
Triticum
Cotyledon
Anthocyanins
In Situ Hybridization, Fluorescence
Chromosome Mapping
Genes, Plant
Description: Published online: 05 January 2021
Rights: © The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature 2021
DOI: 10.1007/s00425-020-03552-6
Appears in Collections:Agriculture, Food and Wine publications
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