Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/129726
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Type: Journal article
Title: Disrupted excitatory synaptic contacts and altered neuronal network activity underpins the neurological phenotype in PCDH19-clustering epilepsy (PCDH19-CE)
Author: Tasheva, S.
Nieto Guil, A.F.
Homan, C.C.
Gecz, J.
Thomas, P.Q.
Citation: Molecular Neurobiology, 2021; 58(5):2005-2018
Publisher: Springer
Issue Date: 2021
ISSN: 0893-7648
1559-1182
Statement of
Responsibility: 
Stefka Mincheva-Tasheva, Alvaro F. Nieto Guil, Claire C. Homan and Jozef Gecz, Paul Q. Thomas
Abstract: PCDH19-Clustering Epilepsy (PCDH19-CE) is an infantile onset disorder caused by mutation of the X-linked PCDH19 gene. Intriguingly, heterozygous females are affected while hemizygous males are not. While there is compelling evidence that this disorder stems from the coexistence of WT and PCDH19-null cells, the cellular mechanism underpinning the neurological phenotype remains unclear. Here, we investigate the impact of Pcdh19 WT and KO neuron mosaicism on synaptogenesis and network activity. Using our previously established knock-in and knock-out mouse models, together with CRISPR-Cas9 genome editing technology, we demonstrate a reduction in excitatory synaptic contacts between PCDH19-expressing and PCDH19-null neurons. Significantly altered neuronal morphology and neuronal network activities were also identified in the mixed populations. In addition, we show that in Pcdh19 heterozygous mice, where the coexistence of WT and KO neurons naturally occurs, aberrant contralateral axonal branching is present. Overall, our data show that mosaic expression of PCDH19 disrupts physiological neurite communication leading to abnormal neuronal activity, a hallmark of PCDH19-CE.
Keywords: Protocadherin 19
PCDH19
PCDH19-Clustering Epilepsy (PCDH19-CE)
Epilepsy
Synapses
Description: Published: 07 January 2021
Rights: © The Author(s), under exclusive licence to Springer Science+Business Media, LLC part of Springer Nature 2021
DOI: 10.1007/s12035-020-02242-4
Grant ID: http://purl.org/au-research/grants/nhmrc/1129679
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