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|Title:||Scavenging of soluble and immobilized CCL21 by ACKR4 regulates peripheral dendritic cell emigration|
|Citation:||Proceedings of the National Academy of Sciences of USA, 2021; 118(17):e2025763118-1-e2025763118-9|
|Publisher:||National Academy of Sciences|
|Cameron R. Bastow, Mark D. Bunting, Ervin E. Kara, Duncan R. McKenzie, Adriana Caon, Sapna Devi, Lynn Tolley, Scott N. Mueller, Ian H. Frazer, Natasha Harvey, Mark R. Condina, Clifford Young, Peter Hoffmann, Shaun R. McColl, and Iain Comerford|
|Abstract:||Leukocyte homing driven by the chemokine CCL21 is pivotal for adaptive immunity because it controls dendritic cell (DC) and T cell migration through CCR7. ACKR4 scavenges CCL21 and has been shown to play an essential role in DC trafficking at the steady state and during immune responses to tumors and cutaneous inflammation. However, the mechanism by which ACKR4 regulates peripheral DC migration is unknown, and the extent to which it regulates CCL21 in steady-state skin and lymph nodes (LNs) is contested. Specifically, our previous findings that CCL21 levels are increased in LNs of ACKR4-deficient mice [I. Comerford et al., <jats:italic>Blood</jats:italic> 116, 4130–4140 (2010)] were refuted [M. H. Ulvmar et al., <jats:italic>Nat. Immunol.</jats:italic> 15, 623–630 (2014)], and no differences in CCL21 levels in steady-state skin of ACKR4-deficient mice were reported despite compromised CCR7-dependent DC egress in these animals [S. A. Bryce et al., <jats:italic>J. Immunol.</jats:italic> 196, 3341–3353 (2016)]. Here, we resolve these issues and reveal that two forms of CCL21, full-length immobilized and cleaved soluble CCL21, exist in steady-state barrier tissues, and both are regulated by ACKR4. Without ACKR4, extracellular CCL21 gradients in barrier sites are saturated and nonfunctional, DCs cannot home directly to lymphatic vessels, and excess soluble CCL21 from peripheral tissues pollutes downstream LNs. The results identify the mechanism by which ACKR4 controls DC migration in barrier tissues and reveal a complex mode of CCL21 regulation in vivo, which enhances understanding of functional chemokine gradient formation.|
|Keywords:||chemokines; atypical chemokine receptors; migration; dendritic cells|
|Description:||Published April 19, 2021.|
|Rights:||© 2021 the Author(s). Published under the PNAS license.|
|Appears in Collections:||Molecular and Biomedical Science publications|
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