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|Title:||Evaluation of the efficiency of Isohelix™ and Rayon swabs for recovery of DNA from metal surfaces|
|Other Titles:||Evaluation of the efficiency of Isohelix TM and Rayon swabs for recovery of DNA from metal surfaces|
|Citation:||Forensic Science Medicine and Pathology, 2021; 17(2):199-207|
|Publisher:||Springer (part of Springer Nature)|
|Dan O. M. Bonsu, Denice Higgins, Julianne Henry, Jeremy J. Austin|
|Abstract:||Purpose We investigated the recovery and extraction efficiency of DNA from three metal surfaces (brass, copper, steel) relevant to forensic casework, and plastic (control) using two different swabbing systems; Rayon and Isohelix™ swabs, with sterile water and isopropyl alcohol respectively, as the wetting solutions. Methods Twenty nanograms of human genomic DNA were applied directly to Isohelix™ and Rayon swabs; and to the metal and plastic substrates. All substrates were left to dry for 24 h, followed by single wet swabbing and extraction with the DNA IQ™ System. DNA extracts were quantified using real time quantitative PCR assays with SYBR green chemistry. Results DNA was extracted from directly seeded Isohelix™ swabs with a high efficiency of 98%, indicating effective DNArelease from the swab into the extraction buffer. In contrast, only 58% of input DNA was recovered from seeded Rayon swabs, indicating higher DNA retention by these swabs. Isohelix™ swabs recovered 32 – 53% of DNA from metal surfaces, whilst the Rayon swabs recovered 11—29%. DNA recovery was lowest from copper and highest from brass. Interestingly, Rayon swabs appeared to collect more DNA from the plastic surface than Isohelix™ swabs, however, due to the lower release of DNA from Rayon swabs they returned less DNA overall following extraction than Isohelix™ swabs. Conclusion These results demonstrate that DNA samples deposited on metal surfaces can be more efficiently recovered using Isohelix™ swabs wetted with isopropyl alcohol than Rayon swabs wetted with sterile water, although recovery is affected by the substrate type.|
|Keywords:||Metal surfaces; Isohelix™ swab; Rayon swab; Collection efficiency; Release efficiency; Extraction efficiency|
|Description:||Published online: 12 November 2020|
|Rights:||© Springer Science+Business Media, LLC, part of Springer Nature 2020|
|Appears in Collections:||Australian Centre for Ancient DNA publications|
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