Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/130277
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dc.contributor.author | Bonsu, D.O.M. | - |
dc.contributor.author | Higgins, D. | - |
dc.contributor.author | Henry, J. | - |
dc.contributor.author | Austin, J.J. | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Forensic Science Medicine and Pathology, 2021; 17(2):199-207 | - |
dc.identifier.issn | 1547-769X | - |
dc.identifier.issn | 1556-2891 | - |
dc.identifier.uri | http://hdl.handle.net/2440/130277 | - |
dc.description | Published online: 12 November 2020 | - |
dc.description.abstract | Purpose We investigated the recovery and extraction efficiency of DNA from three metal surfaces (brass, copper, steel) relevant to forensic casework, and plastic (control) using two different swabbing systems; Rayon and Isohelix™ swabs, with sterile water and isopropyl alcohol respectively, as the wetting solutions. Methods Twenty nanograms of human genomic DNA were applied directly to Isohelix™ and Rayon swabs; and to the metal and plastic substrates. All substrates were left to dry for 24 h, followed by single wet swabbing and extraction with the DNA IQ™ System. DNA extracts were quantified using real time quantitative PCR assays with SYBR green chemistry. Results DNA was extracted from directly seeded Isohelix™ swabs with a high efficiency of 98%, indicating effective DNArelease from the swab into the extraction buffer. In contrast, only 58% of input DNA was recovered from seeded Rayon swabs, indicating higher DNA retention by these swabs. Isohelix™ swabs recovered 32 – 53% of DNA from metal surfaces, whilst the Rayon swabs recovered 11—29%. DNA recovery was lowest from copper and highest from brass. Interestingly, Rayon swabs appeared to collect more DNA from the plastic surface than Isohelix™ swabs, however, due to the lower release of DNA from Rayon swabs they returned less DNA overall following extraction than Isohelix™ swabs. Conclusion These results demonstrate that DNA samples deposited on metal surfaces can be more efficiently recovered using Isohelix™ swabs wetted with isopropyl alcohol than Rayon swabs wetted with sterile water, although recovery is affected by the substrate type. | - |
dc.description.statementofresponsibility | Dan O. M. Bonsu, Denice Higgins, Julianne Henry, Jeremy J. Austin | - |
dc.language.iso | en | - |
dc.publisher | Springer (part of Springer Nature) | - |
dc.rights | © Springer Science+Business Media, LLC, part of Springer Nature 2020 | - |
dc.source.uri | http://dx.doi.org/10.1007/s12024-020-00329-x | - |
dc.subject | Metal surfaces; Isohelix™ swab; Rayon swab; Collection efficiency; Release efficiency; Extraction efficiency | - |
dc.title | Evaluation of the efficiency of Isohelix™ and Rayon swabs for recovery of DNA from metal surfaces | - |
dc.title.alternative | Evaluation of the efficiency of Isohelix TM and Rayon swabs for recovery of DNA from metal surfaces | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1007/s12024-020-00329-x | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Bonsu, D.O.M. [0000-0002-4671-0521] | - |
dc.identifier.orcid | Higgins, D. [0000-0001-7780-243X] | - |
dc.identifier.orcid | Austin, J.J. [0000-0003-4244-2942] | - |
Appears in Collections: | Aurora harvest 8 Australian Centre for Ancient DNA publications |
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File | Description | Size | Format | |
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hdl_130277.pdf | Accepted version | 1.4 MB | Adobe PDF | View/Open |
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