Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Genetic evidence for the vital function of osterix in cementogenesis|
de Crombrugghe, B.
|Citation:||Journal of Bone and Mineral Research, 2012; 27(5):1080-1092|
|Zhengguo Cao, Hua Zhang, Xin Zhou, Xianglong Han, Yinshi Ren, Tian Gao ... et al.|
|Abstract:||To date, attempts to regenerate a complete tooth, including the critical periodontal tissues associated with the tooth root, have not been successful. Controversy still exists regarding the origin of the cell source for cellular cementum (epithelial or mesenchymal). This disagreement may be partially due to a lack of understanding of the events leading to the initiation and development of the tooth roots and supportive tissues, such as the cementum. Osterix (OSX) is a transcriptional factor essential for osteogenesis, but its role in cementogenesis has not been addressed. In the present study, we first documented a close relationship between the temporal‐ and spatial‐expression pattern of Osx and the formation of cellular cementum. We then generated 3.6‐kilobase (kb) collagen type I (3.6‐kb Col 1)‐Osx transgenic mice, which displayed accelerated cementum formation versus wild‐type (WT) controls. Importantly, the conditional deletion of Osx in the mesenchymal cells with two different Cre systems (the 2.3‐kb Col 1 and an inducible CAG–Cre estrogen receptor [CreER]) led to a sharp reduction in cellular cementum formation (including the cementum mass and mineral deposition rate) and gene expression of dentin matrix protein 1 (DMP1) by cementocytes. However, the deletion of the Osx gene after cellular cementum formed did not alter the properties of the mature cementum as evaluated by backscattered scanning electron microscopy (SEM) and resin‐casted SEM. Transient transfection of Osx in the cementoblasts in vitro significantly inhibited cell proliferation and increased cell differentiation and mineralization. Taken together, these data support: (1) the mesenchymal origin of cellular cementum (from periodontal ligament [PDL] progenitor cells); (2) the vital role of OSX in controlling the formation of cellular cementum; and (3) the limited remodeling of cellular cementum in adult mice. © 2012 American Society for Bone and Mineral Research.|
|Keywords:||Osx; cementum; cementogenesis; DMP1; tooth root|
|Rights:||© 2012 American Society for Bone and Mineral Research. Published on behalf of the American Society for Bone and Mineral Research|
|Appears in Collections:||Aurora harvest 8|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.