Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/130425
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: A sexually dimorphic murine model of IUGR induced by embryo transfer
Author: Kaur, H.
Care, A.S.
Wilson, R.L.
Piltz, S.G.
Thomas, P.Q.
Muhlhausler, B.S.
Roberts, C.T.
Gatford, K.L.
Citation: Reproduction, 2021; 161(2):135-144
Publisher: BioScientifica
Issue Date: 2021
ISSN: 0022-4251
1741-7899
Statement of
Responsibility: 
Harleen Kaur, Alison S Care, Rebecca L Wilson, Sandra G Piltz, Paul Q Thomas, Beverly S Muhlhausler ... et al.
Abstract: Animal models are needed to develop interventions to prevent or treat intrauterine growth restriction (IUGR). Foetal growth rates and effects of in utero exposures differ between sexes, but little is known about sex-specific effects of increasing litter size. We established a murine IUGR model using pregnancies generated by multiple embryo transfers, and evaluated sex-specific responses to increasing litter size. CBAF1 embryos were collected at gestation day 0.5 (GD0.5) and 6, 8, 10 or 12 embryos were transferred into each uterine horn of pseudopregnant female CD1 mice (n = 32). Foetal and placental outcomes were measured at GD18.5. In the main experiment, foetuses were genotyped (Sry) for analysis of sex-specific outcomes. The number of implantation sites (P = 0.033) and litter size (number of foetuses, P = 0.008) correlated positively with the number of embryos transferred, while placental weight correlated negatively with litter size (both P < 0.01). The relationship between viable litter size and foetal weight differed between sexes (interaction P = 0.002), such that foetal weights of males (P = 0.002), but not females (P = 0.233), correlated negatively with litter size. Placental weight decreased with increasing litter size (P < 0.001) and was lower in females than males (P = 0.020). Our results suggest that male foetuses grow as fast as permitted by nutrient supply, whereas the female maintains placental reserve capacity. This strategy reflecting sex-specific gene expression is likely to place the male foetus at greater risk of death in the event of a 'second hit'.
Keywords: Placenta
Animals
Mice
Fetal Growth Retardation
Disease Models, Animal
Fetal Weight
Embryo Transfer
Pregnancy
Litter Size
Female
Male
Rights: © 2021 Society for Reproduction and Fertility 2021.
DOI: 10.1530/rep-20-0209
Grant ID: http://purl.org/au-research/grants/nhmrc/1174971
Appears in Collections:Aurora harvest 4
Medicine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.