Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Small RNA, transcriptome and degradome analysis of the transgenerational heat stress response network in durum wheat
Author: Liu, H.
Able, A.J.
Able, J.A.
Citation: International Journal of Molecular Sciences, 2021; 22(11):5532-1-5532-24
Publisher: MDPI AG
Issue Date: 2021
ISSN: 1422-0067
Statement of
Haipei Liu, Amanda J. Able and Jason A. Able
Abstract: Heat stress is a major limiting factor of grain yield and quality in crops. Abiotic stresses have a transgenerational impact and the mechanistic basis is associated with epigenetic regulation. The current study presents the first systematic analysis of the transgenerational effects of post-anthesis heat stress in tetraploid wheat. Leaf physiological traits, harvest components and grain quality traits were characterized under the impact of parental and progeny heat stress. The parental heat stress treatment had a positive influence on the offspring for traits including chlorophyll content, grain weight, grain number and grain total starch content. Integrated sequencing analysis of the small RNAome, mRNA transcriptome and degradome provided the first description of the molecular networks mediating heat stress adaptation under transgenerational influence. The expression profile of 1771 microRNAs (733 being novel) and 66,559 genes was provided, with differentially expressed microRNAs and genes characterized subject to the progeny treatment, parental treatment and tissue-type factors. Gene Ontology and KEGG pathway analysis of stress responsive microRNAsmRNA modules provided further information on their functional roles in biological processes such as hormone homeostasis, signal transduction and protein stabilization. Our results provide new insights on the molecular basis of transgenerational heat stress adaptation, which can be used for improving thermo-tolerance in breeding.
Keywords: heat stress; transgenerational stress memory; next-generation sequencing; microRNAs; cereal reproduction; crop improvement; tetraploid wheat
Description: Published: 24 May 2021
Rights: Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
DOI: 10.3390/ijms22115532
Grant ID:
Published version:
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 8

Files in This Item:
File Description SizeFormat 
hdl_130522.pdfPublished version6.07 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.