Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Omega-3 fatty acids ameliorate vascular inflammation: a rationale for their atheroprotective effects|
Di Bartolo, B.A.
|Citation:||Atherosclerosis, 2021; 324:27-37|
|Anthony D.Pisaniello, Peter J.Psaltis, Peta M.King, Ge Liu, Robert A.Gibson, Joanne TM.Tan ... et al.|
|Abstract:||Background and aims: Clinical trials have demonstrated reductions in major adverse cardiovascular events with purified high-dose eicosapentaenoic acid (EPA), independent of effects on lipids. We aimed to investigate whether omega-3 fatty acids reduce vascular inflammation, a critical mediator of atherosclerosis, and hypothesised that EPA is superior to docosahexaenoic acid (DHA). Methods: In a double-blind randomised controlled trial and cell-culture study, 40 healthy volunteers were supplemented with 4 g daily of either EPA, DHA, fish oil (2:1 EPA:DHA), or placebo for 30 days. Serum was incubated with TNF-stimulated human umbilical vein endothelial cells (HUVECs), and markers of acute vascular inflammation (AVI) were measured. The effects of EPA, DHA (600 mg/kg/day), olive oil, or no treatment were also measured in preclinical models of  AVI using a periarterial collar (C57Bl/6J; n = 40 mice) and  atherosclerosis where ApoE−/− mice (n = 40) were fed a 16-week atherogenic diet. Results: EPA supplementation reduced expression of C–C motif chemokine ligand 2 (CCL2) by 25% compared to placebo (p = 0.03). In the AVI model, EPA reduced vascular expression of VCAM1 by 43% (p = 0.02) and CCL2 by 41% (p = 0.03). Significant inverse correlations were observed between EPA levels and vascular expression of VCAM1 (r = −0.56, p = 0.001) and CCL2 (r = −0.56, p = 0.001). In ApoE−/- mice, EPA reduced aortic expression of Il1b by 44% (p = 0.04) and Tnf by 49% (p = 0.04), with similar inverse correlations between EPA levels and both Il1b (r = −0.63, p = 0.009) and Tnf (r = −0.50, p = 0.04). Conclusions: Supplementation with EPA, more so than DHA, ameliorates acute and chronic vascular inflammation, providing a rationale for the cardiovascular benefit observed with high dose omega-3 fatty acid administration.|
|Keywords:||Omega-3 fatty acids; polyunsaturated fatty acids; lipids; atherosclerosis; vascular inflammation; inflammation; animal models|
|Rights:||© 2021 Elsevier B.V. All rights reserved.|
|Appears in Collections:||Medicine publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.