Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/130630
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Type: Journal article
Title: Development of an Australian bread wheat nested association mapping population, a new genetic diversity resource for breeding under dry and hot climates
Author: Chidzanga, C.
Fleury, D.
Baumann, U.
Mullan, D.
Watanabe, S.
Kalambettu, P.
Pontre, R.
Edwards, J.
Forrest, K.
Wong, D.
Langridge, P.
Chalmers, K.
Garcia, M.
Citation: International Journal of Molecular Sciences, 2021; 22(9):4348-1-4348-18
Publisher: MDPI AG
Issue Date: 2021
ISSN: 1422-0067
1422-0067
Statement of
Responsibility: 
Charity Chidzanga, Delphine Fleury, Ute Baumann, Dan Mullan, Sayuri Watanabe, Priyanka Kalambettu, Robert Pontre, James Edwards, Kerrie Forrest, Debbie Wong, Peter Langridge, Ken Chalmers and Melissa Garcia
Abstract: Genetic diversity, knowledge of the genetic architecture of the traits of interest and efficient means of transferring the desired genetic diversity into the relevant genetic background are prerequisites for plant breeding. Exotic germplasm is a rich source of genetic diversity; however, they harbor undesirable traits that limit their suitability for modern agriculture. Nested association mapping (NAM) populations are valuable genetic resources that enable incorporation of genetic diversity, dissection of complex traits and providing germplasm to breeding programs. We developed the OzNAM by crossing and backcrossing 73 diverse exotic parents to two Australian elite varieties Gladius and Scout. The NAM parents were genotyped using the iSelect wheat 90K Infinium SNP array, and the progeny were genotyped using a custom targeted genotyping-by-sequencing assay based on molecular inversion probes designed to target 12,179 SNPs chosen from the iSelect wheat 90K Infinium SNP array of the parents. In total, 3535 BC₁F₄:₆ RILs from 125 families with 21 to 76 lines per family were genotyped and we found 4964 polymorphic and multi-allelic haplotype markers that spanned the whole genome. A subset of 530 lines from 28 families were evaluated in multi-environment trials over three years. To demonstrate the utility of the population in QTL mapping, we chose to map QTL for maturity and plant height using the RTM-GWAS approach and we identified novel and known QTL for maturity and plant height
Keywords: Nested association mapping; genetic diversity; exotic germplasm; wheat; multi-environmental trials; QTL
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
RMID: 1000040081
DOI: 10.3390/ijms22094348
Grant ID: http://purl.org/au-research/grants/arc/IH130200027
Appears in Collections:Agriculture, Food and Wine publications

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