Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/130648
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Type: Journal article
Title: Cord blood T cells expressing high and low PKCζ levels develop into cells with a propensity to display Th1 and Th9 cytokine profiles, respectively
Other Titles: Cord blood T cells expressing high and low PKCzeta levels develop into cells with a propensity to display Th1 and Th9 cytokine profiles, respectively
Author: Perveen, K.
Quach, A.
McPhee, A.
Prescott, S.L.
Barry, S.C.
Hii, C.S.
Ferrante, A.
Citation: International Journal of Molecular Sciences, 2021; 22(9):4907-1-4907-16
Publisher: MDPI AG
Issue Date: 2021
ISSN: 1422-0067
1422-0067
Statement of
Responsibility: 
Khalida Perveen, Alex Quach, Andrew McPhee, Susan L. Prescott, Simon C. Barry, Charles S. Hii, and Antonio Ferrante
Abstract: Low Protein Kinase C zeta (PKCζ) levels in cord blood T cells (CBTC) have been shown to correlate with the development of allergic sensitization in childhood. However, little is known about the mechanisms responsible. We have examined the relationship between the expression of different levels of PKCζ in CBTC and their development into mature T cell cytokine producers that relate to allergy or anti-allergy promoting cells. Maturation of naïve CBTC was initiated with anti-CD3/-CD28 antibodies and recombinant human interleukin-2 (rhIL-2). To stimulate lymphocyte proliferation and cytokine production the cells were treated with Phytohaemagglutinin (PHA) and Phorbol myristate acetate (PMA). Irrespective of the PKCζ levels expressed, immature CBTC showed no difference in lymphocyte proliferation and the production of T helper 2 (Th2) cytokine interleukin-4 (IL-4) and Th1 cytokine, interferon-gamma (IFN-γ), and influenced neither their maturation from CD45RA+ to CD45RO+ cells nor cell viability/apoptosis. However, upon maturation the low PKCζ expressing cells produced low levels of the Th1 cytokines, IFN-γ, IL-2 and tumour necrosis factor-alpha (TNF), no changes to levels of the Th2 cytokines, IL-4, IL-5 and IL-13, and an increase in the Th9 cytokine, IL-9. Other cytokines, lymphotoxin-α (LT-α), IL-10, IL-17, IL-21, IL-22 and Transforming growth factor-beta (TGF-β) were not significantly different. The findings support the view that low CBTC PKCζ levels relate to the increased risk of developing allergic diseases.
Keywords: Cord blood T cells; T cell maturation; Th1 and Th2 subsets; PKCζ ; cytokines; allergy
Rights: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
RMID: 1000040726
DOI: 10.3390/ijms22094907
Appears in Collections:Paediatrics publications

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