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Type: Journal article
Title: Effects of intermittent fasting or calorie restriction on markers of lipid metabolism in human skeletal muscle
Author: Liu, B.
Hutchison, A.T.
Thompson, C.H.
Lange, K.
Wittert, G.A.
Heilbronn, L.K.
Citation: Journal of Clinical Endocrinology and Metabolism, 2021; 106(3):1-11
Publisher: Oxford University Press
Issue Date: 2021
ISSN: 0021-972X
Statement of
Bo Liu, Amy T. Hutchison, Campbell H. Thompson, Kylie Lange, Gary A. Wittert, and Leonie K. Heilbronn
Abstract: Context: Impaired lipid metabolism is linked with obesity-associated insulin resistance, which may be reversed by caloric restriction (CR). Objective: In a secondary analysis of a randomized controlled trial, we compared the effects of intermittent fasting (IF) and CR on markers of lipid metabolism in muscle. Design: Seventy-six women (BMI 25-40 kg/m²) were randomized to one of three diets for eight weeks and provided with foods at 70% (CR70 and IF70) or 100% (IF100) of energy requirements. IF groups ate breakfast, prior to a 24-hour fast on 3 non-consecutive days per week. On non-fasting days, IF70 ate at 100% and IF100 ate at 145% of energy requirements to achieve the prescribed target. Weight, body composition, insulin sensitivity by clamp, non-esterified fatty acids (NEFA), β-hydroxybutyrate, and markers of lipid metabolism and oxidative stress in muscle by qPCR were measured at baseline and week 8 following a 12-hour overnight fast (all groups) and 24-hour fast (IF groups). Results: IF70 resulted in greater weight and fat losses and reduced NEFA versus CR70 and IF100 after an overnight fast. IF70 and IF100 induced a greater reduction only in mRNA levels of antioxidant enzymes GPX1, SOD1 and SOD2 versus CR70. Fasting for 24-hours increased NEFA and β-hydroxybutyrate in IF groups, but impaired insulin sensitivity and increased PLIN5 mRNA levels. Conclusoins: In comparison to CR, IF did not increase markers of lipid metabolism in muscle, but reduced expression of antioxidant enzymes. However, fasting-induced insulin resistance was detected, alongside increased PLIN5 expression, potentially reflecting transient lipid storage.
Keywords: Calorie restriction; intermittent fasting; lipid metabolism; mitochondria; muscle; obesity
Rights: © The Author(s) 2020. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For permissions, please e-mail:
RMID: 1000026853
DOI: 10.1210/clinem/dgaa707
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Appears in Collections:Medicine publications

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