Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/130722
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Genome-wide association study reveals the genetic complexity of fructan accumulation patterns in barley grain
Author: Matros, A.
Houston, K.
Tucker, M.R.
Schreiber, M.
Berger, B.
Aubert, M.K.
Wilkinson, L.G.
Witzel, K.
Waugh, R.
Seiffert, U.
Burton, R.A.
Citation: Journal of Experimental Botany, 2021; 72(7):2383-2402
Publisher: Oxford University Press
Issue Date: 2021
ISSN: 0022-0957
1460-2431
Statement of
Responsibility: 
Andrea Matros, Kelly Houston, Matthew R. Tucker, Miriam Schreiber, Bettina Berger, Matthew K. Aubert, Laura G. Wilkinson, Katja Witzel, Robbie Waugh, Udo Seiffert, and Rachel A. Burton
Abstract: We profiled the grain oligosaccharide content of 154 two-row spring barley genotypes and quantified 27 compounds, mainly inulin- and neoseries-type fructans, showing differential abundance. Clustering revealed two profile groups where the ‘high’ set contained greater amounts of sugar monomers, sucrose, and overall fructans, but lower fructosylraffinose. A genome-wide association study (GWAS) identified a significant association for the variability of two fructan types: neoseries-DP7 and inulin-DP9, which showed increased strength when applying a novel compound ratio-GWAS approach. Gene models within this region included three known fructan biosynthesis genes (fructan:fructan 1-fructosyltransferase, sucrose:sucrose 1-fructosyltransferase, and sucrose:fructan 6-fructosyltransferase). Two other genes in this region, 6(G)-fructosyltransferase and vacuolar invertase1, have not previously been linked to fructan biosynthesis and showed expression patterns distinct from those of the other three genes, including exclusive expression of 6(G)-fructosyltransferase in outer grain tissues at the storage phase. From exome capture data, several single nucleotide polymorphisms related to inulin- and neoseries-type fructan variability were identified in fructan:fructan 1-fructosyltransferase and 6(G)-fructosyltransferase genes. Co-expression analyses uncovered potential regulators of fructan biosynthesis including transcription factors. Our results provide the first scientific evidence for the distinct biosynthesis of neoseries-type fructans during barley grain maturation and reveal novel gene candidates likely to be involved in the differential biosynthesis of various types of fructan in barley.
Keywords: Barley; expression analysis; fructans; 6G-FFT; grain; GWAS; neoseries; oligosaccharides; ratio-GWAS
Rights: © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com
DOI: 10.1093/jxb/erab002
Grant ID: ARC
Appears in Collections:Agriculture, Food and Wine publications
Aurora harvest 8

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.