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Type: Journal article
Title: Genome-wide association study reveals the genetic complexity of fructan accumulation patterns in barley grain
Author: Matros, A.
Houston, K.
Tucker, M.R.
Schreiber, M.
Berger, B.
Aubert, M.K.
Wilkinson, L.G.
Witzel, K.
Waugh, R.
Seiffert, U.
Burton, R.A.
Citation: Journal of Experimental Botany, 2021; 72(7):2383-2402
Publisher: Oxford University Press
Issue Date: 2021
ISSN: 0022-0957
Statement of
Andrea Matros, Kelly Houston, Matthew R. Tucker, Miriam Schreiber, Bettina Berger, Matthew K. Aubert, Laura G. Wilkinson, Katja Witzel, Robbie Waugh, Udo Seiffert, and Rachel A. Burton
Abstract: We profiled the grain oligosaccharide content of 154 two-row spring barley genotypes and quantified 27 compounds, mainly inulin- and neoseries-type fructans, showing differential abundance. Clustering revealed two profile groups where the ‘high’ set contained greater amounts of sugar monomers, sucrose, and overall fructans, but lower fructosylraffinose. A genome-wide association study (GWAS) identified a significant association for the variability of two fructan types: neoseries-DP7 and inulin-DP9, which showed increased strength when applying a novel compound ratio-GWAS approach. Gene models within this region included three known fructan biosynthesis genes (fructan:fructan 1-fructosyltransferase, sucrose:sucrose 1-fructosyltransferase, and sucrose:fructan 6-fructosyltransferase). Two other genes in this region, 6(G)-fructosyltransferase and vacuolar invertase1, have not previously been linked to fructan biosynthesis and showed expression patterns distinct from those of the other three genes, including exclusive expression of 6(G)-fructosyltransferase in outer grain tissues at the storage phase. From exome capture data, several single nucleotide polymorphisms related to inulin- and neoseries-type fructan variability were identified in fructan:fructan 1-fructosyltransferase and 6(G)-fructosyltransferase genes. Co-expression analyses uncovered potential regulators of fructan biosynthesis including transcription factors. Our results provide the first scientific evidence for the distinct biosynthesis of neoseries-type fructans during barley grain maturation and reveal novel gene candidates likely to be involved in the differential biosynthesis of various types of fructan in barley.
Keywords: Barley; expression analysis; fructans; 6G-FFT; grain; GWAS; neoseries; oligosaccharides; ratio-GWAS
Rights: © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email:
DOI: 10.1093/jxb/erab002
Grant ID: ARC
Appears in Collections:Agriculture, Food and Wine publications
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