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Type: Journal article
Title: Dysregulation of bisphosphoglycerate mutase during in vitro maturation of oocytes
Author: Lim, M.
Brown, H.M.
Rose, R.D.
Thompson, J.G.
Dunning, K.R.
Citation: Journal of Assisted Reproduction and Genetics, 2021; 38(6):1363-1372
Publisher: Springer (part of Springer Nature)
Issue Date: 2021
ISSN: 1058-0468
Statement of
Megan Lim, Hannah M Brown, Ryan D Rose, Jeremy G Thompson, Kylie R Dunning
Abstract: Purpose: Oxygen is vital for oocyte maturation; however, oxygen regulation within ovarian follicles is not fully understood. Hemoglobin is abundant within the in vivo matured oocyte, indicating potential function as an oxygen regulator. However, hemoglobin is significantly reduced following in vitro maturation (IVM). The molecule 2,3-bisphosphoglycerate (2,3-BPG) is essential in red blood cells, facilitating release of oxygen from hemoglobin. Towards understanding the role of 2,3-BPG in the oocyte, we characterized gene expression and protein abundance of bisphosphoglycerate mutase (Bpgm), which synthesizes 2,3-BPG, and whether this is altered under low oxygen or hemoglobin addition during IVM. Methods: Hemoglobin and Bpgm expression within in vivo matured human cumulus cells and mouse cumulus-oocyte complexes (COCs) were evaluated to determine physiological levels of Bpgm. During IVM, Bpgm gene expression and protein abundance were analyzed in the presence or absence of low oxygen (2% and 5% oxygen) or exogenous hemoglobin. Results: The expression of Bpgm was significantly lower than hemoglobin when mouse COCs were matured in vivo. Following IVM at 20% oxygen, Bpgm gene expression and protein abundance were significantly higher compared to in vivo. At 2% oxygen, Bpgm was significantly higher compared to 20% oxygen, while exogenous hemoglobin resulted in significantly lower Bpgm in the COC. Conclusion: Hemoglobin and 2,3-BPG may play a role within the maturing COC. This study shows that IVM increases Bpgm within COCs compared to in vivo. Decreasing oxygen concentration and the addition of hemoglobin altered Bpgm, albeit not to levels observed in vivo.
Keywords: Oocyte maturation
Cumulus-oocyte complex
Bisphosphoglycerate mutase
Description: Published online: 30 May 2021
Rights: © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021
DOI: 10.1007/s10815-021-02230-0
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Obstetrics and Gynaecology publications

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