Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/131079
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Type: Journal article
Title: Effect of oxygen and glucose availability during in vitro maturation of bovine oocytes on development and gene expression
Author: Whitty, A.
Kind, K.L.
Dunning, K.R.
Thompson, J.G.
Citation: Journal of Assisted Reproduction and Genetics, 2021; 38(6):1349-1362
Publisher: Springer
Issue Date: 2021
ISSN: 1058-0468
1573-7330
Statement of
Responsibility: 
Annie Whitty, Karen L. Kind, Kylie R. Dunning and Jeremy G. Thompson
Abstract: PURPOSE: Oxygen tension during the in vitro maturation (IVM) of oocytes is important for oocyte developmental competence. A conflict exists in the literature as to whether low oxygen during IVM is detrimental or beneficial to the oocyte. Many research and clinical labs use higher than physiological oxygen tension perhaps believing that low-oxygen tension is detrimental to oocyte development. Other studies show that glucose is important if low-oxygen tension is used during maturation. In this study, we look at the link between low oxygen and glucose availability during IVM to resolve misconceptions around low-oxygen tension during IVM. METHODS: Bovine cumulus oocyte complexes (COCs) were matured at 20% vs 7% oxygen in media containing differing glucose concentrations or varying availability. Cleavage and blastocyst rates were recorded. RT-PCR determined expression levels of metabolic, oxygen, and stress-responsive genes following IVM. RESULTS: Embryo development in 7% oxygen groups with 2.3mM glucose/low glucose availability was lower than 20% oxygen groups. Under 7% oxygen with 5.6mM glucose or higher glucose availability, rates were restored to those seen in 20% oxygen. Expressions of BNIP3, ENO1, GAPDH, and SLC2A1, were upregulated in 7% oxygen/low glucose, compared to 20% oxygen groups. BNIP3 expression was higher in 7% oxygen group with low glucose availability compared to the 20% groups. CONCLUSION: Oocyte developmental competence is negatively impacted following IVM in low oxygen when glucose availability is limited. Glucose concentration and physical culture conditions need to be considered when comparing the effects of different oxygen concentrations during IVM.
Keywords: Oocyte
Cumulus oocyte complex
In vitro maturation
Bovine
Oxygen
Glucose
BNIP3
ENO1
GAPDH
SLC2A1
Description: Published online: 24 May 2021
Rights: © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021
DOI: 10.1007/s10815-021-02218-w
Grant ID: http://purl.org/au-research/grants/nhmrc/1077694
http://purl.org/au-research/grants/arc/CE140100003
Appears in Collections:Aurora harvest 8
Obstetrics and Gynaecology publications

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