Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/131300
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Structural and functional characterizations of the C-terminal domains of CzcD proteins
Author: Udagedara, S.R.
La Porta, D.M.
Spehar, C.
Purohit, G.
Hein, M.J.A.
Fatmous, M.E.
Casas Garcia, G.P.
Ganio, K.
McDevitt, C.A.
Maher, M.J.
Citation: Journal of Inorganic Biochemistry, 2020; 208:111087-1-111087-9
Publisher: Elsevier
Issue Date: 2020
ISSN: 0162-0134
1873-3344
Statement of
Responsibility: 
Saumya R. Udagedara, Daniel M. La Porta, Christian Spehar, Ghruta Purohit, Matthew J.A. Hein, Monique E. Fatmous, G. Patricia Casas Garcia, Katherine Ganio, Christopher A. McDevitt, Megan J. Maher
Abstract: Zinc is a potent antimicrobial component of the innate immune response at the host-pathogen interface. Bacteria subvert or resist host zinc insults by metal efflux pathways that include cation diffusion facilitator (CDF) proteins. The structural and functional examination of this protein class has been limited, with only the structures of the zinc transporter YiiP proteins from E. coli and Shewanella oneidensis described to date. Here, we determine the metal binding properties, solution quaternary structures and three dimensional architectures of the C-terminal domains of the metal transporter CzcD proteins from Cupriavidus metallidurans, Pseudomonas aeruginosa and Thermotoga maritima. We reveal significant diversity in the metal-binding properties and structures of these proteins and discover a potential novel mechanism for metal-promoted dimerization for the Cupriavidus metallidurans and Pseudomonas aeruginosa proteins.
Keywords: Cation diffusion facilitator protein (CDF); CzcD; zinc; analytical ultracentrifugation (AUC); isothermal titration calorimetry (ITC); X-ray crystallography
Rights: © 2020 Elsevier Inc. All rights reserved.
DOI: 10.1016/j.jinorgbio.2020.111087
Grant ID: http://purl.org/au-research/grants/arc/DP170102102
http://purl.org/au-research/grants/arc/FT180100397
http://purl.org/au-research/grants/arc/FT170100006
http://purl.org/au-research/grants/nhmrc/GNT1080784
http://purl.org/au-research/grants/nhmrc/GNT1140554
Appears in Collections:Aurora harvest 8
Microbiology and Immunology publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.